Cell Signaling Pathways
Cell signaling, often called signal transduction, is a complex system of communication that enables cells to perceive, communicate with and respond to their microenvironment.
Cell signaling may occur in three forms:
- Extracellular signals, such as contact with other cells, chemoattractants, or extracellular matrix (ECM).
- Interaction between cytosolic signaling proteins in response to either intracellular molecules or signals generated from the cytosolic face of transmembrane receptors.
- Nuclear response to stimuli, leading to changes in gene expression.
Kinases in Signaling and Disease
Reversible phosphorylation is the most common regulator of cellular events, regulating an estimated 30% of cellular proteins. Therefore, kinases are intensely studied and frequently targeted for drug development.
The cell`s ability to respond to its environment is essential for normal development, cellular repair and immunity and is the basis of homeostasis. Errors in these pathways, caused by mutations that may result in constitutive expression or total inhibition of protein transcription, are responsible for such diseases as diabetes, autoimmune disorders and cancer.
Your Source for Comprehensive Multiplex Immunoassays
Get the most data from every sample with two powerful MILLIPLEX MAP cell signaling technologies, both using the Luminex xMAP platform:
| MILLIPLEX MAP Cell Signaling Assays | MILLIPLEX MAP EpiQuant Cell Signaling Assays |
|---|---|
| Obtain qualitative data (fold increase or relative concentrations based on lysate standard curves) | Obtain quantitative data (absolute concentrations using synthetic peptides as standards) |
| Measure key signaling pathways with analytically validated, preconfigured kits | Measure multiple phosphorylation sites on the same protein in the same well |
| Build a custom panel by choosing from over 65 MAPmates | Build a custom panel by choosing only the analytes that fit your needs (similar to our soluble panels) |
| Requires multiple wells to measure phospho- and total protein | Measure total and phosphoryated protein in the same well |
| Requires multiple well to measure multiple phosphorylated sites on the same protein | Digest samples using MILLIPLEX MAP EpiQuant Sample Preparation Kit |
| Learn more... | Learn more... |
| New Product Spotlight |
|---|
| Human Phosphorylated STAT Cell Signaling 5 Plex |
| Be able to detect five phosphorylated STAT protein simultaneously with Millipore`s STAT cell signaling panel: a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures. |
The STAT proteins play important roles in:
|
