New SARS-CoV-2 Antigen Rapid Test Kit

The SARS-CoV-2 Antigen Rapid Test Kit (Cat. No: CoV2Ag-25) is used for in vitro qualitative detection of SARS-CoV-2 antigen in human oropharyngeal swabs, nasal swabs and nasopharyngeal swabs.

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Altered concentrations of DKK1 and FGF23 in WNT1 and PLS3 osteoporosis

Recent advancements in genetic research have uncovered new forms of monogenic osteoporosis. Mutations in WNT1, encoding a WNT/β-catenin pathway ligand WNT1, and PLS3, encoding X chromosomally inherited plastin 3 (PLS3), both result in early-onset osteoporosis with prevalent fractures and disrupted bone metabolism. Data from a study by Mäkitie RE et al. indicate a link between PLS3 and DKK1 and WNT1 and FGF23 in bone metabolism (measured with Biomedica kits). Therefore, DKK1 and FGF23 may be clinically useful biomarkers for PLS3 and WNT1 osteoporosis, respectively.

Mäkitie RE, Kämpe A, Costantini A, Alm JJ, Magnusson P, Mäkitie O. Biomarkers in WNT1 and PLS3 osteoporosis: altered concentrations of DKK1 and FGF23. J Bone Miner Res. 2020;35(5):901-912.

Check out the Biomedica DKK-1 and FGF23 ELISA kits.


Validation of Mercodia Lispro NL-ELISA for use in toxicology

The Mercodia Lispro NL-ELISA can reliably be used to measure very high levels of insulin lispro in rat samples. The assay was successfully used by Mercodia in a biosimilar study sponsored by a client.

• Rat insulin up to 3600 pmol/L does not interfere in the specific determination of insulin lispro.
• No matrix effects from rat plasma are observed for the assay.
• Accuracy and precision were well within pre-determined criteria.

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BIOCREDIT COVID-19 Ag

The BIOCREDIT COVID-19 Ag rapid test kit from RapiGEN is a one-step immunochromatographic assay for the qualitative detection of SARS-CoV-2 antigen from nasopharyngeal and/or oropharyngeal swab. Rapid results in 5-8 minutes!

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Mercodia Glucagon ELISA

On October 1st, 2020, Mercodia is launching an updated version of its Glucagon ELISA. The update concerns adjustment to the assay protocol to make certain that even patient samples containing extremely high levels of the proglucagon derivative glicentin give accurate results.

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Blood Cancer Awareness

September is Blood Cancer Awareness month. Every year, more than one million people in the world are newly diagnosed with blood cancers such as leukaemia, lymphoma, and myeloma1. However, many people remain unaware of these often incurable blood cancers, which can develop in individuals of any age, gender or race.

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Big ET - independent predictor of long-term mortality in patients with three-vessel disease

Endothelin-1 (ET-1) is a potent vasoconstrictor secreted by vascular endothelial cells. Due to its high biologic activity, ET-1 is rapidly cleared. However, big ET-1, the precursor of ET-1, has a much longer half-life, circulates in higher concentrations and is easier to detect. Thus, big-ET-1 is a more suitable marker and can be used as an alternative approach for the indirect estimation of ET-1 release. Big ET-1 has been implicated in the pathophysiology of numerous cardiovascular diseases.

Big ET can easily be measured using Biomedica's Big Endothelin-1 ELISA.


The collection of cell markers of various cells

All cells express characteristic markers that can be used to distinguish them from other cell types, such as proteins, lipids and glycosylation. Cell markers can be expressed extracellularly on the cells surface or inside the cell as an intracellular molecule. Cusabio offers surface and intracellular cell markers for a variety of cell types including immune cells, stem cells, central nervous system cells, and more.

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Success of GeneProof SARS-CoV-2 in QCMD

Both GeneProof PCR kits detecting SARS-CoV-2 successfully participated in QCMD panels. GeneProof SARS-CoV-2 PCR Kit (Catno: COV2/GP/100) and GeneProof SARS-CoV-2 Screening PCR Kit (Catno: COV2S/GP/100) succeeded in the pool in the international External Quality Assesment panel.

The PCR kits produced 100% accurate results for all studied parameters. Read press release

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Products for Research on Factor VIII

The Factor VIII antibodies from ImmBioMed, which are members of the renowned ESH series are now available.

• Clone ESH4: detects the light chain of Factor VIII:c (epitope between 2303 – 23332); useful for ELISA reagent
• Clone ESH5: detects the heavy chain of Factor VIII:c; useful for immuno-depletion
• Clone ESH8: detects the light chain of Factor VIII:c; useful for immune-blotting and -depletion; ELISA reagent as coating or conjugate; flow cytometry

LOXO GmbH has compiled a white paper on the historic and current literature on these Factor VIII antibodies, with a special emphasis on their use for flow cytometry. 


Saliva Collection Aid - CE-marked

We can inform you that Salimetrics' Saliva Collection Aids are now CE-marked for the EU. The Saliva Collection Aid is an assisting device that fits securely inside common externally threaded cryovials to simplify collection of whole saliva samples from participants.

In addition, we like to share with you that the recently published book on Salivary Bioscience has been a success in terms of spreading awareness and building critical mass in the field of salivary bioscience.


SARS-CoV-2 S2 N defined peptide pool

Research on SARS-CoV-2 peptides and peptide pools are done intensively showing valuable data on functionality of the virus. Mabtech has developed a specific lyophilized peptide-pool which enables you to find additional answers on the target peptides. The peptide pool is recommended for quantification of the number of cytokine secreting T cells specific for the SARS-CoV-2 spike and nucleoproteins with ELISpot/FluoroSpot. It has been validated with FluoroSpot at Mabtech using human PBMC from COVID-19 convalescent individuals previously PCR-confirmed as SARS-CoV-2 positive.

Order it here


Reading up on Science with our partner Mercodia

Our partner Mercodia is a world-leading developer, manufacturer and distributor of high quality immunoassay kits. They would like to help you make the most of your time, especially with the current situation in the world. Have a look at some of their topics.

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COVID-19 EXBIO Dry Reagent kits

EXBIO decided to face the situation and focus all their effort on communication with medical doctors, clinical cyometry laboratories and researchers to contribute to better understand the course of this disease and finding help to cope with this pandemic. Their research and development teams, in close collaboration with physicians and scientists from clinical cytometric laboratories, designed and developed two Dry Reagent kits for cytometric analysis of T and B cell populations.

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A novel in vitro screening tool of anti-lipidemic drugs

This new publication proposes a new method for in vitro screening of anti-lipidemic drugs: Lipoprotein profile and lipid metabolism of PXB-cells(®), human primary hepatocytes from liver-humanized mice: proposal of novel in vitro system for screening anti-lipidemic drugs. Hata K et al. Biomed Res. 2020;41(1):33-42.

In this study, lipoprotein profile and various factors of lipid metabolism are successfully evaluated using hepatocyte derived from PXB-mouse🄬 a human hepatocyte chimeric mouse, in combination with gel filtration HPLC and ELISA.

Skylight Biotech Inc. 's LipoSEARCH analysis and IBL Japan's ApoB100, ANGPTL3, ANGPTL4 and ANGPTL8 ELISA kits are used in this study.

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Endostatin is an independent risk factor of graft loss after kidney transplant

Researchers have identified the endothelial basement membrane biomarker endostatin that can better predict the risk of graft loss in kidney transplant recipients (KTRs) in a prospective observational cohort study with 574 maintenance KTRs (median follow-up 48 months).

“.. increased serum endostatin concentrations at baseline are independently associated with the risk of graft loss in prevalent KTRs. Its association with graft loss seems to be even stronger as compared to the established classical risk factors for graft loss: decreased GFR and increased urinary protein excretion.“ [reference]

Human endostatin can easily be measured by Biomedica's fully validated Human Endostatin ELISA.


Titin N-Fragment Assay Kit

We are pleased to announce that Titin N-Fragment ELISA (for human sample) from IBL Japan is now available in European market again. Titin-N Fragment is useful tool in research of sport medicine (muscle damage), NAFLD, cardiac disease, sarcopenia and frailty.

Junichi Shoda, MD, PhD, professor of dept. of gastroenterology, Tsukuba University Hospital (Tsukuba, Ibaraki, JAPAN), et al. assessed 153 NAFLD subjects and 100 non NAFLD, obesity, diabetes mellitus subjects in a retrospective study, measured urinary levels of titin-N fragment, a skeletal muscle damage marker, in the possible correlations with NAFLD pathophysiology. In this study, NAFLD subjects had more decreased knee extension strength, greater subcutaneous fat thickness, higher intensity (brightness) of the rectus femoris muscle on ultrasound imaging, and higher levels of the intra- and extra- myocellular lipids in proton magnetic resonance spectroscopy. Urinary titin-N fragment levels were increased with NAFLD subjects compared to non NAFLD, obesity, diabetes mellitus subjects. [reference]

The IBL Titin N-Fragment Assay Kit (CatNo: 27900) was used in this study.


SARS-CoV-2 related products by Cusabio

Cusabio paid close attention to the progression of SARS-CoV-2. Once obtaining the virus gene sequence information, they quickly developed and produced SARS-CoV-2 related products. Targets include the Spike protein (S), N protein (N) and ACE2. Moreover, Cusabio also provides COVID-19 detection kits.

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Notification for TCRB Gene Clonality Assay Kits

IdentiClone® TCRB Gene Clonality Assay - Notification of the Addition of a New Clonal Control DNA

Invivoscribe has announced that their IVS-0021 Clonal Control DNA (4-088-1210) will be included with their IdentiClone® TCRB Gene Clonality Assays.

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Human CD134/OX40 Assay Kit

Factors involved in activated T cells and T cell proliferation / invasion

CD134/OX40 is a receptor of OX40 ligand (OX40L) transactivated by Tax of HTLV-1. OX40 is expressed in activated CD4+ and CD8+ T cells. OX40 on the activated T cells have a role in proliferation of T cells and production of cytokines by binding with OX40L expressed on antigen presenting cells like dendritic cells or activated B cells. In addition, it also be involved in activated T cell invasion to inflamed area through the binding with OX40L.

It has been observed that soluble CD134 / OX40 in plasma is significantly higher in acute adult T-cell leukemia patients compare to HTLV-1 carriers and healthy individual in recent study. It has also been reported that HTLV-1 gene (number of the copy) in peripheral blood lymphocytes was correlated with soluble OX40 level in plasma of HTLV-1 infected patients.

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Exbio HLA-G Antibodies

Understanding the role of HLA-G molecules under normal and pathological situations atracts many researchers and highly specific monoclonal antibodies are needed. Exbio offers a panel of unique antibodies that have been thoroughly validated by organizers of HLA-G workshops and are recommended as reference HLA-G antibodies.

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Mercodia Total GIP NL-ELISA

The Mercodia Total GIP NL-ELISA (CatNo: 10-1258-01) provides a method for the quantitative determination of GIP in human samples.

Mercodia Total GIP NL-ELISA measures active GLP-1(1-42) to the same extent as its metabolite GIP(3-42). Thus, the rapid degradation of the active GIP(1-42) form is not a problem for the specificity of the assay. Accurate results are obtained even if samples without any stabilizing additives are analyzed.

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Assays for the Determination of Human and Mouse Soluble Alpha Klotho (α-Klotho)

Alpha Klotho (α-Klotho) is a key player that integrates a multi-step regulatory system of calcium metabolism and phosphate homeostasis.

Alpha Klotho (α-Klotho) was identified as an extremely down-regulated gene in the genetic mutation mouse (Klotho mouse) of which phenotype is very similar to various symptoms of human aging. Then, the sequences of Alpha Klotho (α-Klotho) genes have been identified in various species including human based on the one of mouse. α-Klotho protein is a 130kDa, one-transmembrane protein and its expression is confirmed in the kidneys and the parathyroid glands. In recent years, it has become clear that α-Klotho is an important molecule within a living organism regulating the metabolism of mineral such as calcium and phosphorus. Therefore, it is considered that, in Klotho mouse, early aging-like symptoms are induced by impaired mineral homeostasis caused by decreased expression of α- Klotho. Meanwhile, it is reported that the long N-terminal extracellular domain which comprises the major portion of sequence of α-Klotho protein is released free into blood by shedding. However, there are may unclear points about functions and changes in concentration of free (soluble) α-Klotho protein, so it has been required to develop the measurement system of α-Klotho.

IBL Japan offers a solid phase sandwich ELISA for the determination of human soluble Alpha Klotho (α-Klotho) in serum and EDTA-plasma and a solid phase sandwich ELISA for the determination of mouse soluble Alpha Klotho (α-Klotho) in mouse urine.


Sun Diagnostics new in our portfolio

Sun Diagnostics simplifies interference testing with the ASSURANCE™ product line. The ASSURANCE™ interferents are high quality, highly concentrated materials from human sources (where possible) or purified chemicals, and are available as kit or individual vials to best match users’ needs.

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Mercodia Lispro NL-ELISA

Mercodia is launching a new ELISA assay for the insulin analog Lispro. This easy-to-use, high quality PK assay meets the pharmaceutical industry requirements for multiple phases of drug development.

• Optimized for pharma
• Calibrators in serum matrix
• Fully EMA/FDA validated for human and porcine samples
• Sample volume: 10 µL
• Detection method: Chemiluminescence

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Immune Checkpoint Assays – Screen your immune checkpoint blockers

The HTRF immune checkpoint binding assays are designed to measure the interaction between immune checkpoint proteins.

The best TR FRET assays on the market
• All components included: validated proteins, detection antibodies & buffers
• Screen human or mouse blocker antibodies
• Screen small molecules
• From 96 to 1536 format

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Bio-Connect is supporting member of World Wide Cancer Research

Worldwide Cancer Research funds lifesaving cancer research projects in the best research institutions in the UK and across the world. It funds exceptional researchers who work 24 hours a day, seven days a week. It does all of those things for one very good reason - to gain a global perspective. Because research doesn’t happen in isolation. The answers will not come from one scientist, in one lab, in one country.

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Cytokines Related Products from Elabscience

Elabscience provides high-quality and cost-effective reagents related to cytokines, including chemokines, interferons, interleukins, lymphokines, and tumour necrosis factors.

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Four Types of ELISA

ELISA, short for enzyme-linked immunosorbent assay, is a very mature method for the detection of various targets. One advantage of ELISA is that it's quick and simple to carry out, so it is often used for both diagnostic and research purposes.

As its name implies, ELISA involves the use of enzymes and the specific binding of antibody and antigen. According to how it works, ELISA can be divided into four major types: direct, indirect, sandwich, and competitive. Let's see them one by one.

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4' video to set up cAMP assays with HTRF

A protocol video from Cisbio Bioassays (HTRF®) to introduce you to cAMP assays with HTRF® technology. Watch this video to learn about utilizing a streamlined, no wash, add and read HTRF® cAMP assay kit to monitor GPCR activation.

Features
• A presentation of the kit and assay principle
• Explanations and illustrated guidelines
• Results interpretation


Alternative Protocol for Mercodia Glucagon ELISA

The Mercodia Glucagon ELISA is highly specific and shows very low cross-reactivity to oxyntomodulin and glicentin.

But, under certain conditions like
• Post bariatric surgery
• Kidney disease
• Exogenous administration

…the levels of glicentin, proglucagon 1-61 and oxyndomodulin can be elevated to levels that interfere in the Glucagon ELISA. To avoid these interferences, an alternative protocol has been developed.

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Three outstanding application notes to discover the HTRF α-tubulin housekeeping kit

α-tubulin proteins are commonly used for housekeeping, and measuring their levels is crucial for determining the action of a compound’s mechanisms.

The new HTRF α-tubulin housekeeping kit is based on the TR-FRET immunoassay format and measures the endogenous level of α-tubulin in cell and tissue lysates.

Cisbio has prepared three application notes for you that include:
• Case studies for multi-parametric detection in the same cell lysate
• Demonstrations of kit adaptability to different species, buffers, cellular types, and cultures
• Evidence of this kit’s advantages over other kits

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LymphoTrack® Dx TRB Assay on MiSeq®

Invivoscribe has completed their CE-marked IVD LymphoTrack® Dx Assay suite with the addition of the LymphoTrack Dx TRB Assay.

The LymphoTrack Dx TRB Assay - MiSeq® is an in vitro diagnostic product intended to identify and track clonal T-cell receptor (TRB) gene rearrangements using next-generation sequencing (NGS) on the Illumina® MiSeq®. This assay will determine the frequency distribution of TRB Vβ-Jβ gene rearrangements in patients suspected of having lymphoproliferative disease.

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Early diagnosis of osteoporosis

New evidence: microRNA bone biomarkers can improve early diagnosis of osteoporosis.
First comprehensive attempt to model the cost-effectiveness of circulating microRNAs for bone fracture risk assessment in comparison with DXA and FRAX®.

Cost-utility analysis of fracture risk assessment using microRNAs compared with standard tools and no monitoring in the Austrian female population. E. Walter, H. Dellago, J. Grillari, H.P. Dimai, M. Hackl, Bone, Volume 108, 2018, pages 44-54. Click link for full-text publication.

The results demonstrate that the osteomiR™ kit can be a cost-effective alternative to established risk assessment strategies.

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Elabscience neuroscientific reagents

Are you working in the field of neuroscience? Please have a look at the high quality ELISA kits and antibodies from Elabscience for the study associated with the nervous system.

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Euro Diagnostica new in our portfolio

Bio-Connect Diagnostics is proud to inform you that we have become partner of Euro Diagnostica. We are now the distributor of Euro Diagnostica in The Netherlands. Euro Diagnostica provides clinical labs and physicians with tools to aid in diagnosis, prognosis, monitoring and treatment of inflammatory and related diseases. By offering high quality diagnostic assays.

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GeneProof MRSA PCR kit

Bio-Connect Diagnostics would like to inform you about launch of GeneProof's new product the MRSA PCR kit. The PCR kit is designed for methicillin-resistant Staphylococcus aureus detection by the real-time Polymerase Chain Reaction (PCR) method.

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The Only Internationally Standardized CE-IVD Assay for FLT3 Signal Ratio Mutation Analysis

The LeukoStrat CDx FLT3 Mutation Assay from Invivoscribe is a PCR-based in vitro diagnostic test designed to detect internal tandem duplications (ITD), tyrosine kinase domain (TKD) mutations (D835 and I836) and to determine the respective signal ratio (SR) values in the FLT3 gene. This assay amplifies genomic DNA extracted from mononuclear cells obtained from peripheral blood or bone marrow aspirates of patients diagnosed with acute myelogenous leukemia (AML).

Companion Diagnostics Novartis and Invivoscribe »»

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Revel the Mechanisms of Host Defense by Cross-regulation of TLR Responses

A recent Nature Immunology paper describes the epigenetic landscape that results from the integration of the inflammatory cytokines type I interferons and TNF.

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Elabscience antibodies available on our website

Elabscience provides a wide range of antibodies, including over 20,000 polyclonal and monoclonal antibodies, phospho antibodies, acetyl antibodies, tag antibodies, secondary antibodies, isotype antibodies, etc. All antibodies are strictly tested to ensure the purity and quality. Elabscience's antibodies can be applied in ELISA, WB, IHC, IP and ChIP.

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GeneProof Factor XIII V34L PCR kit

The GeneProof Factor XIII V34L PCR kit is designed to detect polymorphism of the F13A1 gene encoding the subunit A of FXIII factor by use of the real-time Polymerase Chain Reaction (PCR) method.

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Procollagen-III-peptide (PIIINP) ELISA kit

Bio-Connect Diagnostics would like to inform you about the launching of Cisbio's IVD P3NP-ELISA kit. The first ELISA P3NP CE-marked kit available on the market.

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NEW LeukoStrat FLT3 Assay - Available Now!

Invivoscribe has released their CE-marked LeukoStrat® FLT3 Mutation Assay 2.0. This in vitro diagnostic (IVD) product is intended for PCR-based ABI detection of FLT3 activating mutations in patients with acute myelogenous leukemia (AML).

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Introducing GeneProof

Bio-Connect Diagnostics is proud to introduce GeneProof and its products to the Benelux market. They are a biotechnology company operating in the field of molecular in vitro diagnostics of serious infectious and genetic diseases. Their portfolio includes PCR kits, DNA and RNA extraction kits, and nucleic acid extraction system. All GeneProof products are CE IVD certified.

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Mercodia Total GLP-1 NL-ELISA

We are proud to present the new Mercodia Total GLP-1 NL-ELISA, the first assay in their new chemiluminescent product line “Northern Lights”. The Total GLP-1 ELISA requires only 25 µL of human plasma and the dynamic range is 0.9-940 pmol/L.

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Cisbio HTRF®

HTRF®, is the premier TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) technology on the market. TR-FRET technologies such as HTRF® bring together the sensitivity of fluorescence with the homogeneous nature of FRET (Fluorescence Resonance Energy Transfer) and the low background of time resolution. HTRF® uses two fluorophores, a donor and an acceptor dye, that transfer energy when in close proximity to each other. This creates a homogeneous assay format in which bound and unbound partners do not need to be separated as fluorescence emission from the acceptor is generated only upon binding.

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CE-Marked NGS-Based LymphoTrack® Dx Assays

Invivoscribe has released four CE-marked LymphoTrack® Dx next-generation sequencing (NGS) assays used to detect T-cell receptor gamma (TRG) and immunoglobulin heavy chain (IGH) gene rearrangements. These in vitro diagnostic (IVD) test kits, validated for use with the Illumina MiSeq® and ThermoFisher Ion PGM™ instruments, comprise just a few of the CE-marked LymphoTrack Dx assays that Invivoscribe intends to release.

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Exclusive for Mercodia products

Bio-Connect Diagnostics B.V. has become the exclusive partner of Mercodia products in the Benelux. We have intensified our cooperation to offer you better service, good technical support and easier ways to order.

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FGF23 (C-terminal) ELISA - CE marked!

Biomedica launches first CE-marked FGF23 ELISA assay in Europe and Asia
Vienna, Austria, April 22, 2015 / B3C newswire / -- The biomarker specialists at Biomedica Immunoassays, announced the launch of the first CE marked FGF23 (C-terminal) ELISA assay which specifically detects both intact and C-terminal fragments of FGF23 in human serum and plasma. The FGF23 ELISA assay complements the existing Biomedica assays directed against chronic kidney disease - mineral and bone disorder (CKD-MBD) in a growing collection. The FGF23 ELISA assay will be an important tool for researchers investigating new biomarkers that could improve prediction of CKD progression, as well as discovery of new drug targets.

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Ghrelin EIA kits

Bertin Pharma offers a totally new set of assays that will allow any scientist to measure acylated and unacylated Ghrelin whatever the collection procedure. Those assays are called Easy Sampling because any blood sample collected can be used on any kind of protease inhibitors with or without acidification. While there is not yet a consensus on the collection procedure, we are quite confident that the scientific community will work toward this, especially if the biomarker is involved in clinical trials or later on in clinic. It is today largely accepted that a study involving Ghrelin should include both acylated and unacylated forms, while total Ghrelin is almost irrelevant.

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Introducing the Mercodia Glucagon ELISA – 10µL

The Mercodia Glucagon ELISA – 10µL is based on our very popular Glucagon ELISA, but requires an even lower sample volume. The need for high sample volumes in glucagon assays has for a long time limited the possibility of performing time-course studies of glucagon levels when working with rodents as a model animals.

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Next-Generation Sequencing products

We are excited to announce that Invivoscribe has expanded the portfolio of next-generation sequencing (NGS) products for both the Illumina MiSeq® and Life Technologies / Thermo Fisher Ion PGM™ platforms with the introduction of three additional assays which completes a suite of assays which allows testing of TRG and IGH:

• LymphoTrack® IGH Somatic Hypermutation Assay – PGM
• LymphoTrack® IGH + TRG Assay – PGM
• LymphoTrack® IGH + TRG Assay – MiSeq

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