A novel in vitro screening tool of anti-lipidemic drugs

Lipoprotein profile and lipid metabolism analysis using fresh human hepatocytes isolated from PXB-Mouse­čäČ

Keishi Hata et al, a senior researcher at Akita Research Institute of Food & Brewing, Akita, Japan, investigated lipid metabolism in fresh human hepatocytes isolated from PXB-Mouse­čäČ, a human hepatocyte chimeric mouse, conducting RT-PCR, ELISA and gel-filtration HPLC to reveal mRNA expressions and secreted protein levels and lipoprotein profile in the cultured medium. As a result, they found 1) these cells produce higher levels of lipoproteins compared to HepG2 and Huh-7, 2) produce lipoprotein mainly in VLDL fraction very similarily to human primary hepatocytes. 3) When added fenofibrate they responded to produce lesser lipoproteins in dose dependent manner, and also 4) to increase mRNA and secreted protein levels of ANGPTL4.

Lipoprotein profile and lipid metabolism of PXB-cells(®), human primary hepatocytes from liver-humanized mice: proposal of novel in vitro system for screening anti-lipidemic drugs. Hata K et al. Biomed Res. 2020;41(1):33-42.

So far researches have been made in applications of this PXB-mouse­čäČ derived hepatocyte in drug metabolism and hepatitis virus infection. Contrary to these fields, applications of these cells in lipid/sugar metabolism were studied to lesser degree.

This report concludes that a combination of this new human hepatocyte and analysis of lipoprotein and lipid metabolism can be a useful method for in vitro screening of anti-lipidemic drugs.

In this report Lipoprotein profile analysis with gel-filtration HPLC (LipoSEARCH­čäČ) are performed and IBL's ELISAs below are used to measure ApoB100, ANGPTL3,4,8 in cultured mediums.

Cat. No.Description
Human ApoB-100 Assay Kit
Human ANGPTL3 (highly sensitive) Assay Kit
Human ANGPTL4 Assay Kit
Human ANGPTL8 Assay Kit

These products are for research use only and it cannot be used for diagnosis or medical purpose.

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Brands: IBL Japan