Acylated and Unacylated Ghrelin EIA kits

This Enzyme Immunometric Assay (EIA) is based on a double-antibody sandwich technique. The wells of the plate supplied with the kit are coated with a monoclonal antibody specific to the C-terminal part of ghrelin. This antibody will bind to any ghrelin introduced into the wells (standard or sample). The acetylcholinesterase (AChE) - Fab' conjugate which recognises the N-terminal part of acylated ghrelin is also added to the wells. This allows the two antibodies to form a sandwich by binding on different parts of the human acylated ghrelin.

The sandwich is immobilised on the plate so the excess reagents may be washed away. The concentration of the human acylated ghrelin is then determined by measuring the enzymatic activity of the immobilized AChE using the Ellman's Reagent. The AChE tracer acts on the Ellman's Reagent to form a yellow compound.

The intensity of the colour, which is determined by spectrophotometry, is proportional to the amount of the human acylated ghrelin present in the well during the immunological incubation.

Ghrelin EIA Kits

Cat. No.DescriptionAmountBrandCategory
A05106Acylated Ghrelin (human) Express EIA kit
96 wells
Bertin Bioreagent
Assays
A05106
Acylated Ghrelin (human) Express EIA kit
384 wells
Bertin Bioreagent
Assays
A05117
Acylated Ghrelin (mouse, rat) Express EIA kit
96 wells
Bertin Bioreagent
Assays
A05118
Unacylated Ghrelin (mouse, rat) Express EIA kit
96 wells
Bertin Bioreagent
Assays
A05119Unacylated Ghrelin (human) Express EIA kit
96 wells
Bertin Bioreagent
Assays
A05317
Acylated Ghrelin (mouse, rat) Easy Sampling EIA kit
96 wells
Bertin Bioreagent
Assays
A05318Unacylated Ghrelin (mouse, rat) Easy Sampling EIA kit
96 wells
Bertin Bioreagent
Assays
A05319Unacylated Ghrelin (human) Easy Sampling EIA kit
96 wells
Bertin Bioreagent
Assays
A05320Unacylated Ghrelin (dog) Easy Sampling EIA kit
96 wells
Bertin Bioreagent
Assays
A05321Acylated Ghrelin (dog) Easy Sampling EIA kit
96 wells
Bertin Bioreagent
Assays
A05401
Acylated Ghrelin (pig) EIA kit
96 wells
Bertin Bioreagent
Assays
A05402Unacylated Ghrelin (pig) EIA kit
96 wells
Bertin Bioreagent
Assays

Related product

Cat.No.DescriptionAmountBrandCategory
D31009
Sampling tubes with PHMB
35 tubes
Bertin Bioreagent
Labware / Accessories




Source:
Brochure: Ghrelin

Related to:
Brands: Bertin Bioreagent
Product groups: Assays


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