Immunohistochemical analysis of paraffin-embedded human colon, using Myosin light chain kinase Antibody(M01697) MYLK was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MYLK Antibody (M01697)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-Myosin light chain kinase Rabbit Monoclonal Antibody
M01697
Product group Proteins / Signaling Molecules
Overview
- SupplierBoster Bio
- Product NameAnti-Myosin light chain kinase Rabbit Monoclonal Antibody
- Delivery Days Customer9
- CertificationResearch Use Only
- UNSPSC12352202