IHC analysis of ANP using anti-ANP antibody (A01318-1). ANP was detected in paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-ANP Antibody (A01318-1) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-ANP/Nppa Antibody
A01318-1
Product group Antibodies
Overview
- SupplierBoster Bio
- Product NameAnti-ANP/Nppa Antibody
- Delivery Days Customer9
- CertificationResearch Use Only
- UNSPSC12352203