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Description
Catalog number
CAS No.
Clone ID
News
Vorige
Volgende
Anti-CD52 Monoclonal Antibody
Catalog number:
M03484
Brand:
Boster Bio
Packing:
100 ug
Price:
On Request
Expected delivery time:
10 days
Quantity:
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General
Pictures
Figure 1. Western blot analysis of ...
Figure 1. Western blot analysis of Cd52 using anti-Cd52 antibody (M03484).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cd52 antigen affinity purified polyclonal antibody (Catalog # M03484) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for Cd52.
Product specifications for - Anti-CD52 Monoclonal Antibody
Overview:
Product group:
Antibodies
Category:
Primary Antibodies
Species:
Mouse
Host:
Rabbit
Clonality:
Monoclonal
Properties:
Datasheet:
Datasheet
Research Use Only
UNSPSC:
12352203
Form supplied:
Liquid
Storage instructions:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4-¦C short term. Store at -20-¦C long term. Avoid freeze / thaw cycle.
Scientific information:
Scientific info:
Rabbit IgG Monoclonal antibody for Q64389 detection. Tested positive for WB IP in Mouse
Clone ID:
AEIF-3
Swiss prot ID:
Q64389
Additional information:
Synonyms:
M03484