IHC analysis of Collagen IV using anti-Collagen IV antibody (M01411). Collagen IV was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Collagen IV Antibody (M01411) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Anti-Collagen IV Antibody (monoclonal, 3G3)
M01411
Product group Antibodies
Overview
- SupplierBoster Bio
- Product NameAnti-Collagen IV Antibody (monoclonal, 3G3)
- Delivery Days Customer9
- CertificationResearch Use Only
- UNSPSC12352203