IHC analysis of Creatine Kinase MM using anti-Creatine Kinase MM antibody (A03452-1). Creatine Kinase MM was detected in paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Creatine Kinase MM Antibody (A03452-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-Creatine Kinase MM/CKM Antibody
A03452-1
Product group Proteins / Signaling Molecules
Overview
- SupplierBoster Bio
- Product NameAnti-Creatine Kinase MM/CKM Antibody
- Delivery Days Customer9
- CertificationResearch Use Only
- UNSPSC12352202