IHC analysis of GZMB using anti-GZMB antibody (M00353-1) on paraffin-embedded human T cell lymphoma. GZMB was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GZMB Antibody (M00353-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
IHC analysis of GZMB using anti-GZMB antibody (M00353-1) on paraffin-embedded human T cell lymphoma. GZMB was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GZMB Antibody (M00353-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-Granzyme B Monoclonal Antibody
M00353-1
Product group Antibodies
Overview
- SupplierBoster Bio
- Product NameAnti-Granzyme B Monoclonal Antibody
- Delivery Days Customer9
- CertificationResearch Use Only
- UNSPSC12352203