IHC analysis of Leptin using anti-Leptin antibody (A00479-4). Leptin was detected in paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Leptin Antibody (A00479-4) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-Leptin Antibody
A00479-4
Product group Antibodies
Overview
- SupplierBoster Bio
- Product NameAnti-Leptin Antibody
- Delivery Days Customer9
- CertificationResearch Use Only
- UNSPSC12352203