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Western blot analysis of TRAF3 using anti-TRAF3 antibody (A00424). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 cell lysates, Lane 2: rat brain tissue lysates, Lane 3: rat PC-12 cell lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse HEPA1-6 cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAF3 antigen affinity purified polyclonal antibody (Catalog # A00424) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAF3 at approximately 60,64KD. The expected band size for TRAF3 is at 64KD.
Western blot analysis of TRAF3 using anti-TRAF3 antibody (A00424). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 cell lysates, Lane 2: rat brain tissue lysates, Lane 3: rat PC-12 cell lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse HEPA1-6 cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAF3 antigen affinity purified polyclonal antibody (Catalog # A00424) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAF3 at approximately 60,64KD. The expected band size for TRAF3 is at 64KD.
Western blot analysis of TRAF3 using anti-TRAF3 antibody (A00424). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 cell lysates, Lane 2: rat brain tissue lysates, Lane 3: rat PC-12 cell lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse HEPA1-6 cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAF3 antigen affinity purified polyclonal antibody (Catalog # A00424) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAF3 at approximately 60,64KD. The expected band size for TRAF3 is at 64KD.

Anti-TRAF3 Antibody

Research Use Only
A00424
Boster Bio
Product group Antibodies
Price on request
100 ug/vial
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-TRAF3 Antibody
  • Delivery Days Customer
    9
  • Certification
    Research Use Only
  • UNSPSC
    12352203