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Description
Catalog number
CAS No.
Clone ID
News
Vorige
Volgende
Anti-Tyrosinase Rabbit Monoclonal Antibody
Catalog number:
M00326-1
Brand:
Boster Bio
Packing:
100 ug/vial
Price:
On Request
Expected delivery time:
10 days
Quantity:
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General
Pictures
Western blot analysis of Tyrosinase...
Western blot analysis of Tyrosinase expression in human melanoma lysate (M00326-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TYR monoclonal antibody (Catalog # M00326-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TYR
Product specifications for - Anti-Tyrosinase Rabbit Monoclonal Antibody
Overview:
Product group:
Antibodies
Category:
Primary Antibodies
Species:
Human
Host:
Rabbit
Clonality:
Monoclonal
Properties:
Molecular Formula:
60393
Datasheet:
Datasheet
Research Use Only
UNSPSC:
12352203
Form supplied:
Liquid
Storage instructions:
At -20-¦C for one year. After reconstitution, at 4-¦C for one month. It can also be aliquotted and stored frozen at -20-¦C for a longer time. Avoid repeated freezing and thawing.
Scientific information:
Scientific info:
Anti-Tyrosinase Rabbit Monoclonal Antibodytested for IF, IHC, ICC, WB in Human
Clone ID:
AAHE-20
Swiss prot ID:
P14679
Additional information:
Synonyms:
M00326-1