The minimum detectable dose of this kit is typically less than 43,5pg/mL
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Serum, plasma and other biological fluids;
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2,5 hours
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123,46-10000pg/mL: The standard curve concentrations used for the ELISAs were 10000pg/mL 3333,33pg/mL 1111,11pg/mL 370,37pg/mL 123,46pg/mL
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The test principle applied in this kit is Sandwich ELISA: enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 1,25-Dihydroxyvitamin D3 (DHVD3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 1,25-Dihydroxyvitamin D3 (DHVD3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only tho
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Datasheet
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Research Use Only
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41116158
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