The minimum detectable dose of this kit is typically less than 4,38pg/mL
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This assay has high sensitivity and excellent specificity for detection of Estradiol (E2). No significant cross-reactivity or interference between Estradiol (E2) and analogues was observed.
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Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids;
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2,5 hours
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12,35-1000pg/mL: The standard curve concentrations used for the ELISAs were 1000pg/mL 333,33pg/mL 111,11pg/mL 37,04pg/mL 12,35pg/mL
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This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific for Estradiol (E2) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between Horseradish Peroxidase (HRP) labeled Estradiol (E2) and unlabeled Estradiol (E2) (Standards or samples) with the pre-coated antibody specific for Estradiol (E2). After incubation the unbound conjugate is washed off. The amount of bound HRP conjugate is reverse proportional to the concentration of Estra
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Datasheet
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Research Use Only
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41116158
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