The minimum detectable dose of this kit is typically less than 0,54ug/mL
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This assay has high sensitivity and excellent specificity for detection of Glutathione (GSH). No significant cross-reactivity or interference between Glutathione (GSH) and analogues was observed.
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Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids;
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2,5 hours
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1,23-100ug/mL: The standard curve concentrations used for the ELISAs were 100ug/mL 33,33ug/mL 11,11ug/mL 3,7ug/mL 1,23ug/mL
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This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific for Glutathione (GSH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between Horseradish Peroxidase (HRP) labeled Glutathione (GSH) and unlabeled Glutathione (GSH) (Standards or samples) with the pre-coated antibody specific for Glutathione (GSH). After incubation the unbound conjugate is washed off. The amount of bound HRP conjugate is reverse proportional to the concentrat
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Datasheet
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Research Use Only
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41116158
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