The minimum detectable dose of this kit is typically less than 0,057ng/mL
|
Plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids;
|
4,5 hours
|
0,156-10ng/mL: The standard curve concentrations used for the ELISAs were 10ng/mL 5ng/mL 2,5ng/mL 1,25ng/mL 0,625ng/mL 0,313ng/mL 0,156ng/mL
|
The test principle applied in this kit is Sandwich ELISA: enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Differentiation Factor 1 (GDF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Growth Differentiation Factor 1 (GDF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is add
|
Datasheet
|
Research Use Only
|
41116158
|