ELISA Kit for Macrophage Inflammatory Protein 1 Beta (MIP1b)

Product specifications for - ELISA Kit for Macrophage Inflammatory Protein 1 Beta (MIP1b)

Overview:
Product group:	Assays
Category:	ELISA / EIA
Target:	CCL4
Target description:	chemokine (C-C motif) ligand 4

Properties:
Assay Sensitivity:	The minimum detectable dose of this kit is typically less than 5,5pg/mL
Assay Sample Type:	Serum, plasma and other biological fluids;
Assay Time:	4,5 hours
Assay Detection Range:	15,625-1000pg/mL: The standard curve concentrations used for the ELISAs were 1000pg/mL 500pg/mL 250pg/mL 125pg/mL 62,5pg/mL 31,25pg/mL 15,625pg/mL
Assay Test Principle:	The test principle applied in this kit is Sandwich ELISA: enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Inflammatory Protein 1 Beta (MIP1b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Macrophage Inflammatory Protein 1 Beta (MIP1b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate
Datasheet:	Datasheet
	Research Use Only
UNSPSC:	41116158

Scientific information:
Scientific info:	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. Procedure: 1. Prepare all reagents, samples and standards, 2. Add 100 uL standard or sample to each well. Incubate 2 hours at 37°C, 3. Aspirate and add 100 uL prepared Detection Reagent A. Incubate 1 hour at 37°C, 4. Aspirate and wash 3 times, 5. Add 100 uL prepared Detection Reagent B. Incubate 30 minutes at 37°C, 6. Aspirate and wash 5 times, 7. Add 90 uL Substrate Solution. Incubate 15-25 minutes at 37°C, 8. Add 50 uL Stop Solution. Read at 450nm immediately.
Gene ID:	6351

Scientific information:

Scientific info:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. Procedure: 1. Prepare all reagents, samples and standards, 2. Add 100 uL standard or sample to each well. Incubate 2 hours at 37°C, 3. Aspirate and add 100 uL prepared Detection Reagent A. Incubate 1 hour at 37°C, 4. Aspirate and wash 3 times, 5. Add 100 uL prepared Detection Reagent B. Incubate 30 minutes at 37°C, 6. Aspirate and wash 5 times, 7. Add 90 uL Substrate Solution. Incubate 15-25 minutes at 37°C, 8. Add 50 uL Stop Solution. Read at 450nm immediately.

Gene ID:

6351

Additional information:
Synonyms:	SEA093Hu; CCL4L1; CCL4; SCYA4; SCYA2; MIP1B1; MIP1B; MIP-1-beta; LAG1; LAG-1; HC21; G-26; AT744.1; ACT2; small inducible cytokine A4 (homologous to mouse Mip-1b); secreted protein G-26; macrophage inflammatory protein 1-beta; lymphocyte activation gene 1 protein; T-cell activation protein 2; SIS-gamma; PAT 744; MIP-1-beta(1-69); G-26 T-lymphocyte-secreted protein; C-C motif chemokine 4; chemokine (C-C motif) ligand 4

Additional information:

Synonyms:

SEA093Hu; CCL4L1; CCL4; SCYA4; SCYA2; MIP1B1; MIP1B; MIP-1-beta; LAG1; LAG-1; HC21; G-26; AT744.1; ACT2; small inducible cytokine A4 (homologous to mouse Mip-1b); secreted protein G-26; macrophage inflammatory protein 1-beta; lymphocyte activation gene 1 protein; T-cell activation protein 2; SIS-gamma; PAT 744; MIP-1-beta(1-69); G-26 T-lymphocyte-secreted protein; C-C motif chemokine 4; chemokine (C-C motif) ligand 4

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Product specifications for - ELISA Kit for Macrophage Inflammatory Protein 1 Beta (MIP1b)