The minimum detectable dose of this kit is typically less than 0,054ng/mL
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Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids;
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4,5 hours
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0,156-10ng/mL: The standard curve concentrations used for the ELISAs were 10ng/mL 5ng/mL 2,5ng/mL 1,25ng/mL 0,625ng/mL 0,313ng/mL 0,156ng/mL
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The test principle applied in this kit is Sandwich ELISA: enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Monocyte Chemotactic Protein 1 (MCP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Monocyte Chemotactic Protein 1 (MCP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added
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Datasheet
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Research Use Only
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41116158
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