ELISA Kit for Transforming Growth Factor Beta 1 (TGFb1)

Product specifications for - ELISA Kit for Transforming Growth Factor Beta 1 (TGFb1)

Overview:
Product group:	Assays
Category:	ELISA / EIA
Target:	TGFB1
Target description:	transforming growth factor, beta 1

Properties:
Assay Sensitivity:	The minimum detectable dose of this kit is typically less than 12,5pg/mL
Assay Sample Type:	Serum, platelet-poor plasma, tissue homogenates, cell culture supernates and other biological fluids;
Assay Time:	4,5 hours
Assay Detection Range:	31,25-2000pg/mL: The standard curve concentrations used for the ELISAs were 2000pg/mL 1000pg/mL 500pg/mL 250pg/mL 125pg/mL 62,5pg/mL 31,25pg/mL
Assay Test Principle:	The test principle applied in this kit is Sandwich ELISA: enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transforming Growth Factor Beta 1 (TGFb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transforming Growth Factor Beta 1 (TGFb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution
Datasheet:	Datasheet
	Research Use Only
UNSPSC:	41116158

Scientific information:
Scientific info:	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. Procedure: 1. Prepare all reagents, samples and standards, 2. Add 100 uL standard or sample to each well. Incubate 2 hours at 37°C, 3. Aspirate and add 100 uL prepared Detection Reagent A. Incubate 1 hour at 37°C, 4. Aspirate and wash 3 times, 5. Add 100 uL prepared Detection Reagent B. Incubate 30 minutes at 37°C, 6. Aspirate and wash 5 times, 7. Add 90 uL Substrate Solution. Incubate 15-25 minutes at 37°C, 8. Add 50 uL Stop Solution. Read at 450nm immediately.
Gene ID:	7040

Scientific information:

Scientific info:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. Procedure: 1. Prepare all reagents, samples and standards, 2. Add 100 uL standard or sample to each well. Incubate 2 hours at 37°C, 3. Aspirate and add 100 uL prepared Detection Reagent A. Incubate 1 hour at 37°C, 4. Aspirate and wash 3 times, 5. Add 100 uL prepared Detection Reagent B. Incubate 30 minutes at 37°C, 6. Aspirate and wash 5 times, 7. Add 90 uL Substrate Solution. Incubate 15-25 minutes at 37°C, 8. Add 50 uL Stop Solution. Read at 450nm immediately.

Gene ID:

7040

Additional information:
Synonyms:	SEA124Hu; TGF beta 1; TGFB1 / TGF Beta; TGFB1; TGFbeta; TGFB; LAP; DPD1; CED; transforming growth factor beta-1; latency-associated peptide; TGF-beta-1; TGF-beta 1 protein; transforming growth factor, beta 1

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Product specifications for - ELISA Kit for Transforming Growth Factor Beta 1 (TGFb1)