The minimum detectable dose of this kit is typically less than 12,5pg/mL
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Serum, platelet-poor plasma, tissue homogenates, cell culture supernates and other biological fluids;
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4,5 hours
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31,25-2000pg/mL: The standard curve concentrations used for the ELISAs were 2000pg/mL 1000pg/mL 500pg/mL 250pg/mL 125pg/mL 62,5pg/mL 31,25pg/mL
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The test principle applied in this kit is Sandwich ELISA: enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transforming Growth Factor Beta 1 (TGFb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transforming Growth Factor Beta 1 (TGFb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution
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Datasheet
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Research Use Only
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41116158
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