The minimum detectable dose of this kit is typically less than 5,5pg/mL
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Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids;
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4,5 hours
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15,625-1000pg/mL: The standard curve concentrations used for the ELISAs were 1000pg/mL 500pg/mL 250pg/mL 125pg/mL 62,5pg/mL 31,25pg/mL 15,625pg/mL
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The test principle applied in this kit is Sandwich ELISA: enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Alpha (TNFa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Necrosis Factor Alpha (TNFa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only
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Datasheet
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Research Use Only
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41116158
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