EZ4U - Cell Proliferation and Cytotoxicity Assay

Catalog number: BI-5000
Brand: Biomedica
Packing: 10 x 96 wells
Price: On Request
Expected delivery time: 7 days

Product specifications for - EZ4U - Cell Proliferation and Cytotoxicity Assay

Product group: Assays
Category: Cell based assays
Subcategory: Proliferation
Application note: colorimetric assay
Assay Sample Type: cell ulture medium
Assay Time: 2-5 hours, depending on the metabolic capacity of the cells
Datasheet: Datasheet
  Research Use Only
UNSPSC: 41116133
Scientific information: 
Scientific info: Product Characteristics: The EZ4U assay is a non-radioactive, non-toxic cell proliferation and cytotoxicity assay. The assay set-up is performed in a manner similar to the standard 3H-thymidine incorporation method. Instead of pulsing with tritiated nucleotide, 20 ul of dye solution is added to 200 ul sample. Incubation time is dependent on the metabolic capacity of the cells. Usually 2 to 5 hours of incubation at 37°C are sufficient to yield a significant increase in colour intensity which can be measured with a normal microplate-reader at 450nm. The EZ4U system is well suited for a variety of biological tests, where cell viability is of importance. It offers advantages over conventional dye or 3H incorporation assays. Due to its soluble end products it is easier and faster to perform than other non-radioactive cell viability assays. Furthermore, because the assay procedure is identical to the thymidine incorporation procedure, no changes in test protocols are necessary. An important benefit is the possibility of an ongoing cultivation after cell number determination and easy to adapt incubation times due to the non-toxic substrate.
Safety information: 
Additional information: 
Synonyms: BI-5000; Biomedica Gruppe
Plangger A et al., “Cytotoxicity of combinations of the pan-KRAS inhibitor BAY-293 against primary non-small lung cancer cells.” Transl Oncol. (2021); Dec;14(12):101230. Read more
Salesa B et al., “Zinc Chloride: Time-Dependent Cytotoxicity, Proliferation and Promotion of Glycoprotein Synthesis and Antioxidant Gene Expression in Human Keratinocytes.” Biology (Basel). (2021); Oct 20;10(11):1072. Read more
Moschovaki Filippidou F et al., “Glucagon-Like Peptide-1 Receptor Agonism Improves Nephrotoxic Serum Nephritis by Inhibiting T-Cell Proliferation.” Am J Pathol. (2020); Feb;190(2):400-411. Read more
All Citations Read more