The GeneProof PCR kit is designed for BK and JC virus detection and differentiation by the real-time Polymerase Chain Reaction (PCR) method.
The BK/JC detection and differentiation are based on the principle of amplifying the specific conservative DNA sequence overlapping the boundary between the gene for the VP1 and VP2 proteins and measuring the concentration increase of the amplification product during the PCR process using fluorophore labelled probes. BK virus presence is indicated by FAM fluorophore fluorescence growth and the JC virus presence is indicated by Cy5 fluorophore fluorescence growth. An Internal Standard (IS) is either included in the reaction mix, controlling the possible inhibition of the PCR (ISIN version) or excluded, controlling also the DNA extraction process quality (ISEX version). IS positive amplification is detected in the HEX fluorophore fluorescence channel. The detection kit utilizes the hot start technology minimizing non-specific reactions and assuring maximum sensitivity. Ready to Use MasterMix contains uracil-DNA-glycosylase (UDG) eliminating possible contamination of the PCR reaction by amplification products. The kit performs very sensitive BK and JC viruses detection in clinical material (EDTA whole blood, urine, cerebrospinal fluid). The kit is designed for in vitro diagnostics and provides qualitative and quantitative detection.
|