GeneProof Bordetella pertussis/parapertussis PCR Kit

Catalog number: BP/ISEX/100
Brand: GeneProof
Packing: 100 reactions
Other sizes: Other sizes available
Price: On Request
Expected delivery time: 7 days

Product specifications for - GeneProof Bordetella pertussis/parapertussis PCR Kit

Product group: Assays
Category: PCR assay
  CE and IVD Certification
UNSPSC: 41106302
Storage instructions: Store at -20C
Scientific information: 
Scientific info: The GeneProof PCR kit is designed for the detection and differentiation of Bordetella pertussis and Bordetella parapertussis by the real-time Polymerase Chain Reaction (PCR) method.

Bordetella detection and differentiation is based on the amplification of the multi-copy insertion sequence IS1002 (specific for both Bordetella species) and the multi-copy insertion sequence IS1001 (specific only for B. parapertussis) and measuring the growth in the amplification product concentration using PCR and fluorophore labelled probes. One of the few kits in the market that do not provide false positive results for Bordetella holmesii. B. pertussis presence is indicated by the FAM fluorophore fluorescence growth and at the same time by the absence of fluorescence in the Cy5 channel. B. parapertussis presence is indicated by the fluorescence growth in FAM and Cy5 channels. An Internal Standard (IS) is either included in the reaction mix, controlling the possible inhibition of the PCR (ISIN version) or excluded, controlling also the DNA extraction process quality (ISEX version). IS positive amplification is detected in the fluorescence channel for the HEX fluorophore. The detection kit utilizes the hot start technology, minimizing non-specific reactions and assuring maximum sensitivity. Ready to Use MasterMix contains uracil-DNA-glycosylase (UDG), eliminating possible contamination of the PCR reaction by amplification products. The kit performs very sensitive Bordetella detection in clinical material (sputum, nasal aspirate, nasopharyngeal aspirate). The kit is designed for in vitro diagnostics and provides qualitative detection.
Additional information: 
Synonyms: BP/ISEX; GeneProof