The GeneProof PCR kit is designed for Chlamydia pneumoniae detection by the real-time Polymerase Chain Reaction (PCR) method.
The C. pneumoniae detection is based on the amplification of a specific conservative DNA sequence of a single-copy ompA gene and measuring the amplification product concentration using PCR process and fluorophore labelled probes. C. pneumoniae presence is indicated by FAM fluorophore fluorescence growth. An Internal Standard (IS) is either included in the reaction mix, controlling the possible inhibition of the PCR (ISIN version) or excluded, controlling also the DNA extraction process quality (ISEX version). IS positive amplification is detected in the HEX fluorophore fluorescence channel. The detection kit utilizes the hot start technology, minimizing non-specific reactions and assuring maximum sensitivity. Ready to Use MasterMix contains uracil-DNA-glycosylase (UDG), eliminating possible contamination of the PCR reaction by amplification products. The kit performs very sensitive C. pneumoniae detection in clinical material (sputum, bronchoalveolar lavage, nasopharyngeal swab, nasopharyngeal aspirate). The kit is designed for in vitro diagnostics and provides qualitative detection.
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