The GeneProof PCR kit is designed for detection of human Cytomegalovirus by the real-time Polymerase Chain Reaction (PCR) method.
Detection is based on the amplification of a specific conservative DNA sequence of a single-copy gene encoding the 4 IE antigen and measuring the amplification product concentration using PCR process and fluorophore labelled probes. CMV presence is indicated by FAM fluorophore fluorescence growth. An Internal Standard (IS) is either included in the reaction mix, controlling the possible inhibition of the PCR (ISIN version) or excluded, controlling also the DNA extraction process quality (ISEX version). IS positive amplification is detected in the HEX fluorophore fluorescence channel. The detection kit utilizes the hot start technology, minimizing nonspecific reactions and assuring maximum sensitivity. Ready to Use MasterMix contains uracil-DNA-glycosylase (UDG), eliminating possible contamination of the PCR reaction by amplification products. The kit performs very sensitive CMV detection in clinical material (EDTA whole blood, EDTA plasma, cerebrospinal fluid, bone marrow, stool, urine, serum). The kit is designed for in vitro diagnostics and provides qualitative and quantitative detection.
|