The minimum detectable dose of this kit is typically less than 0,59pg/mL
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Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids;
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4,5 hours
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1,563-100pg/mL: The standard curve concentrations used for the ELISAs were 100pg/mL 50pg/mL 25pg/mL 12,5pg/mL 6,25pg/mL 3,125pg/mL 1,563pg/mL
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The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Tumor Necrosis Factor Alpha (TNFa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color
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Datasheet
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Research Use Only
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41116158
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