Human TNF-alpha(Tumor Necrosis Factor Alpha) ELISA Kit

Catalog number: E-EL-H0109
Brand: Elabscience
Packing: 96 tests
Other sizes: 48 tests also available, please inquire.
Price: € 556.00
Expected delivery time: 14 days
Standard Curve

Product specifications for - Human TNF-alpha(Tumor Necrosis Factor Alpha) ELISA Kit

Product group: Assays
Category: ELISA / EIA
Species: Human
Assay Sensitivity: 4.688 pg/mL
Assay Sample Type: Serum, Plasma, Biological Fluids
Assay Detection Range: 7.813-500 pg/mL
Datasheet: Datasheet
  Research Use Only
UNSPSC: 41116158
Scientific information: 
Scientific info: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TNF-α. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TNF-α and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TNF-α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TNF-α. The concentration of Human TNF-α in samples can be calculated by comparing the OD of the samples to the standard curve.
Swiss prot ID: P01375
Additional information: 
Synonyms: E-EL-H0109; Elabscience; Tumor Necrosis Factor Alpha