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Human VEGF ELISA

Catalog number: BI-VEGF
Brand: Biomedica
Packing: 98 tests
Price: On Request
Expected delivery time: 7 days
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Product specifications for - Human VEGF ELISA

Overview: 
Product group: Assays
Category: ELISA / EIA
Application note: ELISA
Species: Human
Reactivity note: human
Properties: 
Molecular weight: 19.20 kDa
Assay Sensitivity: 2.5 pg/ml
Assay Specificity: Vascular endothelial growth factor, VEGF-A
Assay Precision: intra-assay: 3%, inter-assay: 6%
Assay Sample Type: Serum, EDTA plasma, heparin plasma, citrate plasma, urine, cell culture supernatant
Assay Time: 2 h / 1 h / 1 h / 30 min
Assay Detection Range: 31.25-2000 pg/ml
Datasheet: Datasheet
  Research Use Only
UNSPSC: 41116133
Scientific information: 
Scientific info: Product Characteristics: The Biomedica VEGF Sandwich ELISA kit is a test that is intended for the quantitative measurement of VEGF levels in human serum and plasma samples. Target information: Vascular endothelial growth factor (VEGF or VEGF-A), is a growth hormone secreted by endothelial cells, fibroblasts, smooth muscle cells, platelets, macrophages, and many other cell types. It belongs to the cysteine-knot growth factor superfamily (Peach C et al. ) and has a molecular weight of about 40 kDa. Up to date 17 different VEGF isoforms were described to be expressed from one single gene. They are produced by alternative promoter usage/initiation or alternative splicing/proteolysis after protein translation. The N-terminal region is responsible for receptor binding and conserved among all VEGF isoforms. In contrast, residues of the C-terminus differ between isoforms and determine protein length and properties: binding to co-receptor Neuropilin-1 (NRP1) or to extracellular matrix (ECM), agonist/antagonist of angiogenesis. Most isoforms result from the common transcripts: VEGF111, VEGF121, VEGF145, VEGF165, VEGF189 and VEGF206. Additionally, a third VEGF variant (VEGFAx), that demonstrates pro- and anti-apoptotic properties, was described. Thus, vascularization is tightly controlled by the balance of various splice variants, their availability and concentration, whereas isoforms linked to the ECM constitute a reservoir of VEGF that can quickly be shed to circulating forms (Peach C et al.). One of the most potent pro-angiogenic isoforms is VEGF165a. After secretion, 50-70% of VEGF165a is attached to the extracellular matrix (via heparin binding site), the rest is freely diffusible ( Peach C et al.). It is the most abundant isoform and enhances signaling over the VEGFR2 receptor by additionally binding to its co-receptor Neuropilin-1. VEGF A isoforms are glycosylated, homodimeric proteins. Two anti-parallel monomers are linked by intermolecular disulfide bonds (Wiesmann C et al.), whereas eight cysteine residues form a knot-like structure at one end of each monomer (Tjwa M et al.). However, heterodimerization with PLGF has been described as well (Olsson AK et al.)
Gene ID: 7422
Swiss prot ID: P15692
Additional information: 
Synonyms: BI-VEGF; Biomedica Gruppe