Mouse ERα (Estrogen Receptor Alpha) ELISA Kit | E-EL-M0476 | Elabscience

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Product specifications for - Mouse ERalpha(Estrogen Receptor Alpha) ELISA Kit

Overview:
Product group:	Assays
Category:	ELISA / EIA
Species:	Mouse

Properties:
Assay Sensitivity:	0.188ng/mL
Assay Sample Type:	Serum, Plasma, Biological Fluids
Assay Detection Range:	0.313-20ng/mL
	Research Use Only
UNSPSC:	41116158

Scientific information:
Scientific info:	This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ERα. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ERα and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ERα, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse ERα. The concentration of Mouse ERα in samples can be calculated by comparing the OD of the samples to the standard curve.

Scientific information:

Scientific info:

This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ERα. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ERα and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ERα, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse ERα. The concentration of Mouse ERα in samples can be calculated by comparing the OD of the samples to the standard curve.

Additional information:
Synonyms:	E-EL-M0476; Elabscience; Estrogen Receptor Alpha

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Mouse ERalpha(Estrogen Receptor Alpha) ELISA Kit

Product specifications for - Mouse ERalpha(Estrogen Receptor Alpha) ELISA Kit