NT-proANP ELISA

Catalog number: BI-20892
Brand: Biomedica
Packing: 96 tests
Price: On Request
Expected delivery time: 7 days
Quantity:

Product specifications for - NT-proANP ELISA

Overview: 
Product group: Assays
Category: ELISA / EIA
Application note: ELISA
Reactivity note: human
Properties: 
Molecular weight: 12.7 kDa
Assay Sensitivity: 0.05 nmol/l (= 0.64 ng/ml)
Assay Specificity: NT-proANP (equivalent to proANP 1-98), (N-terminal pro-Atrial Natriuretic Peptide). Assay suitable for rat and mouse samples.
Assay Precision: intra-assay: 5%, inter-assay: 9%
Assay Sample Type: Serum, EDTA plasma, heparin plasma, cell culture supernatant and urine samples
Assay Time: 3 h / 30 min
Assay Detection Range: 0.63-10 nmol/l
Datasheet: Datasheet
  Research Use Only
UNSPSC: 41116133
Scientific information: 
Scientific info: Product Characteristics: The Biomedica NT-proANP Sandwich ELISA kit is a test that is intended for the quantitative measurement of N-terminal atrial natriuretic peptide levels in human, dog, rat, or mouse serum, EDTA plasma, heparin plasma, cell culture supernatant and urine samples. The Biomedica NT-proANP ELISA is an independently validated test with >130 NT-proANP ELISA specific publications to date, CE certified and approved for in-vitro diagnistic use in the EU. The Biomedica NT-proANP ELISA was also successfully used in pre-clinical rat studies of pharmaceutical companies due to the highly conserved sequence between human and rat NT-proANP.Target information: Atrial natriuretic peptide is synthesized in atrial myocytes and is stored in secretory granules as a 126 amino acid prohormone. The most important stimulus for the release of the hormone into circulation is stretch of the myocyte fibres. On release the prohormone is split into equimolar amounts of the highly biologically active proANP (99-126), also known as alpha-ANP, and the N-terminal part proANP (1-98). alpha-ANP is rapidly cleared from the circulation with a half-life of 3-4 minutes. NT-proANP (1-98) has a much longer half-life (60-120 min) which leads to significantly higher concentrations in blood compared to alpha-ANP. Thus, circulating levels of NT-proANP (1-98) are less sensitive to the pulsatile secretion of ANP and may better reflect chronic levels than the rapidly fluctuating levels of alpha-ANP. NT-proANP is discussed as valuable marker for e.g. sepsis (Increased plasma levels of NT-proANP and NT-proBNP as markers of cardiac dysfunction in septic patients.), or risk stratification in heart failure (Neurohormonal risk stratification for sudden death and death owing to progressive heart failure in chronic heart failure).
Gene ID: 4878
Swiss prot ID: P01160
Safety information: 
MSDS: MSDS
Additional information: 
Synonyms: BI-20892; Biomedica Gruppe; ANF; ANP; ATFB6; ATRST2; CDD; CDD-ANF; CDP; natriuretic peptide; natriuretic peptide A; NPPA; NT proANP; PND; proANP
Romaniello A et a., “Circulating NT-proANP level is a predictor of mortality for systemic sclerosis: a retrospective study of an Italian cohort.” Expert Rev Clin Immunol. (2021); Jun;17(6):661-666. Read more
Vinken P et al., “Cross-laboratory analytical validation of the cardiac biomarker NT-proANP in rat.” J Pharmacol Toxicol Methods. (2016): 77:58-65. Read more
Zhang K et al., „ The infarction zone rather than the noninfarcted remodeling zone overexpresses angiotensin II receptor type 1 and is the main source of ventricular atrial natriuretic peptide.” Cardiovasc Pathol. (2020); 44:107160. Read more
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