Pregnenolone?ELISA?Kit

Catalog number: E-EL-0086
Brand: Elabscience
Packing: 96 tests
Other sizes: 48 tests also available, please inquire.
Price: € 556.00
Expected delivery time: 14 days
Quantity:
Standard Curve

Product specifications for - Pregnenolone?ELISA?Kit

Overview: 
Product group: Assays
Category: ELISA / EIA
Species: All Species
Properties: 
Assay Sensitivity: 0.469ng/mL
Assay Sample Type: Serum, Plasma, Biological Fluids
Assay Detection Range: 0.781-50ng/mL
  Research Use Only
UNSPSC: 41116158
Scientific information: 
Scientific info: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Pregnenolone. During the reaction, Pregnenolone in the sample or standard competes with a fixed amount of Pregnenolone on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Pregnenolone. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Pregnenolone in the samples is then determined by comparing the OD of the samples to the standard curve.
Additional information: 
Synonyms: E-EL-0086; Elabscience