Diabetes ELISAs

Diabetes mellitus is a medical disorder characterized by elevated blood glucose levels (hyperglycemia). If blood glucose exceeds levels that the body is able to utilize, excess sugar is secreted in the urine. Increased amounts of urine are then produced and the patient becomes very thirsty. Untreated diabetes can lead to dehydration and ketoacidosis. Although the cause of diabetes may differ, all types of diabetes share the same symptoms and late stage complications, such as cardiovascular disease, renal failure, retinal damage with eventual blindness, nerve damage and tissue necrosis (diabetic foot). Read more »»

Human ELISA

Lindström et al. (2002) combined the Insulin ELISA (detects human insulin only) with the Iso-Insulin ELISA (detects human insulin as well as the insulin analogs) in studies of insulin pharmacokinetics in type 1 diabetic patients. Craig et al. (2003) used the Ultrasensitive C-peptide ELISA in studies of the correlation of blood C-peptide levels and occurrence of enterovirus RNA in children with type 1 diabetes. They found that patients with high C-peptide levels were significantly more likely to be negative for enterovirus RNA. Pellmé et al. (2003) found an inverse correlation of proinsulin to adiponectin in first-degree relatives of type 2 diabetic patients by using the Proinsulin ELISA.

Cat. No.
DescriptionSample Volume
Range
10-1136-01
C-peptide ELISA
25 µL
100 - 4000 pmol/L
10-1141-01
Ultrasensitive C-peptide ELISA
50 µL
5 - 280 pmol/L
10-1273-01
Glicentin ELISA
25 µL
3 - 300 pmol/L
10-1271-01
Glucagon ELISA
25 µL
1.5-130 pmol/L
10-1113-01/10
Insulin ELISA
25 µL
3 - 200 mU/L
10-1132-01
Ultrasensitive Insulin ELISA
25 µL
0.15 - 20 mU/L
10-1128-01
Iso-Insulin ELISA
25 µL
3 - 100 mU/L
10-1118-01
Proinsulin ELISA
50 µL
3.3 - 132 pmol/L

Rat and Mouse ELISA

The Rat Insulin ELISAs and Mouse Insulin ELISAs have been used in several in vivo studies of rat and mouse models as well as in in vitro studies of rat and mouse derived cell and tissue cultures. Blyszczuk et al. (2003) used the Mouse Insulin ELISA to monitor insulin secretion from embryonic tem cells differentiated into insulin producing cells. Transplantation of these cells into diabetic mice resulted in a normalization of blood glucose levels. Jaeckel et al. 2004) monitored insulin production in transgenic mice by measuring insulin levels in organ extracts with the Ultrasensitive Mouse Insulin ELISA. Burcelin and colleagues (2003) used the Ultrasensitive Mouse Insulin ELISA on plasma samples from transgenic mice in their studies of GLUT4, AMP kinase and the insulin receptor. Kullin et al. (2003) used the High Range Insulin ELISA to monitor insulin release from isolated rat pancreatic islets pretreated with the potassium channel openers diazoxide or NNC 55-0118 before exposure to the beta cell-suppressive substances alloxan, sodium nitroprusside or IL-1b. Buettner and colleagues (2006) studied rats and the impact of various diets with different fat constitution on insulin secretion and other plasma characteristics by using the Rat Insulin ELISA.

Cat. No.
DescriptionSample Volume
Range
10-1172-01
Rat C-peptide ELISA
10 µL
100 - 4000 pmol/L
10-1275-01
Rat Glicentin ELISA
10 µL
5 - 500 pmol/L
10-1281-01
Glucagon ELISA - 10 ul
10 µL
2-180 pmol/L
10-1247-01/10
Mouse Insulin ELISA
10 µL
0.2 - 6.5 µg/L
10-1249-01
Mouse Ultrasensitive Insulin ELISA
25 µL
0.025 - 1.5 µg/L
10-1250-01/10
Rat Insulin ELISA
10 µL
0.15 - 5.5 µg/L
10-1251-01
Rat Ultrasensitive Insulin ELISA
25 µL
0.02 - 1 µg/L
10-1248-10
Rat/Mouse Insulin FIA
10 µL
0.15 - 100 µg/L
10-1232-01
Rat/Mouse Proinsulin ELISA
25 µL
3.5 - 200 pmol/L

Animal ELISA

Animal models and in vitro cell and tissue cultures from larger animals are widely used in diabetic research as well as in insulin monitoring within veterinary medicine. For example in islet transplantation, the shortage of human donors and difficulty in isolating purified islets from human pancreas has drawn attention to the possibility of xenotransplantation with porcine pancreatic islets. Furthermore, extensive research is conducted on insulin resistance, diabetes mellitus and how to improve the nutritional, metabolic and health status of ruminants, horses and companion animals. Matching energy intake with the energy expenditure is important for the exercising horse. Diabetes mellitus is one of the most common endocrine disorders in dogs. Mercodia provides species specific optimized ELISA assays for measurements of insulin in Porcine, Bovine, Ovine, Canine, and Equine samples, all valuable tools for your insulin measurement quality.

Cat. No.
DescriptionFormatRange
10-1256-01
Porcine C-peptide ELISA
1 x 96 wells
20 - 1200 pmol/L
10-1271-01
Glucagon ELISA
1 x 96 wells
1.5 - 130 pmol/L
10-1281-01
Glucagon ELISA - 10 ul
1 x 96 wells
2 - 180 pmol/L
10-1201-01
Bovine Insulin ELISA
1 x 96 wells
0.05 - 3.0 µg/L
10-1203-01
Canine Insulin ELISA
1 x 96 wells
0.02 - 1.5 µg/L
10-1205-01
Equine Insulin ELISA
1 x 96 wells
0.02 - 1.5 µg/L
10-1233-01
Feline Insulin ELISA
1 x 96 wells
10 - 700 ng/L
10-1202-01
Ovine Insulin ELISA
1 x 96 wells
0.05 - 3.0 µg/L
10-1200-01
Porcine Insulin ELISA
1 x 96 wells
0.02 - 1.5 µg/L



Source:
Brochure: Diabetes Products

Related to:
Brands: Mercodia
Product groups: Assays