Cisbio Bioassays (HTRF®)

HTRF®, is the premier TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) technology on the market. TR-FRET technologies such as HTRF® bring together the sensitivity of fluorescence with the homogeneous nature of FRET (Fluorescence Resonance Energy Transfer) and the low background of time resolution. HTRF® uses two fluorophores, a donor and an acceptor dye, that transfer energy when in close proximity to each other. This creates a homogeneous assay format in which bound and unbound partners do not need to be separated as fluorescence emission from the acceptor is generated only upon binding.

HTRF® can be used in competitive and non-competitive formats and performed as cellular or biochemical assays in 96-, 384- and 1536-well plate formats. It has been applied to a variety of applications including GPCRs, kinases, epigenetics, biotherapeutics and quantification of a range of biomarkers including cytokines.

Bio-Connect Diagnostics supplies all Cisbio HTRF® products in the Benelux. You can find the products in our search engine. If you have any questions, please contact our techsupport.

Major product lines

• Assays for measuring phosphorylated and total proteins directly in cell
• Biomarkers and cytokines
• Biotherapeutics
• Epigenetic screening
• Kinases and cellular phospho-/total protein assays
• Kits and reagents to address the whole GPCR activation pathway
• Receptor binding assays
• Toolbox products and services

Cisbio news

• Immune checkpoint assays »»
• Alpha-tubulin housekeeping cellular kit »»

Cat. No.	Description	Amount
62ASMAPEG	Alpha-SMA kit	500 tests
62AM4PEB	cAMP - Gs Dynamic kit	1000 tests
62AM6PEB	cAMP - Gs HiRange kit	1000 tests
62DL5DDC	Diluent 5 (5X)	10 ml
62EZBFDD	Enzymatic buffer kinase 5X	50 ml
62EL1FDH	Epigeneous lysis buffer A	10000 tests
63ADK045PEG	FcRn kit	100 tests
63ADK036PEG	FLAG check kit	500 tests
SHALOTBC	HaloTag-Lumi4-Tb	2 nmol
62HTMDADA	Histamine - Dynamic kit - acylation reagent	1 each
66PL96025	HTRF 96 well low volume plate	25 plates
62SDBRDD	HTRF detection buffer	40 ml
62SDBRDF	HTRF KinEASE detection kit buffer	200 mL
62ST2PEB	HTRF KinEASE -STK S2 kit	1000 tests
62TK0PEC	HTRF KinEASE-TK kit	20000 tests
63ADK053PEG	Human c-Myc cell-based kit	500 tests
62HIL1BPEH	Human IL1 beta kit	10000 tests
62AMXADC	IBMX - Phosphodiesterase inhibitor	500 ul
62IPAPEB	IP-One	1000 tests
62CL1FDD	Lysis & Detection Buffer 1	13 ml
64CL8FDG	Lysis & Detection Buffer 8	200 ml
61HISDLB	MAb Anti 6HIS-d2	20000 tests
61FG2KLA	MAb Anti FLAG M2-Eu cryptate	5000 tests
61FG2XLA	MAb Anti FLAG M2-XL665	5000 tests
61GSTTLA	MAb Anti GST-Tb cryptate	5000 tests
61KB0KAE	Mab Anti histone H3K9 Me0-Eu(K)	500 tests
62SAHCLB	Methyltransferase assay calibrator	1 each
L0040RED	Muscarinic M1 receptor red antagonist	1 each
PCLIP5HT2C	pCLIP-5HT2C 5-Hydroxytryptamine 2C	1 each
62PG2CDA	Prostaglandin E2 kit standard	1 each
62RB2RDD	Reconstitution buffer 2	13 ml
62RB3RDE	Reconstitution buffer 3	50 ml
61SEBALB	SEB - Suppl. Enzymatic Buffer	12,5 nmol
LABMED	SNAP/CLIP lab medium 5X	100 ml
SSNPTBC	SNAP-Lumi4-Tb	2 nmol
62AT3PEH	STAT3 phospho-Y705 kit	10000 tests
61ST1BLC	STK-Substrate 1-biotin	500 ug
610SADLB	Streptavidin-d2	20000 tests
610SATLB	Streptavidin-Tb cryptate	20000 tests
610SAXLA	Streptavidin-XL665	5000 tests
C1TT1A2A	Tag-lite A2A labeled cells	200 tests
C2SU1DOP	Tag-lite Delta opioid cell line (CHO)	1 each
C1TT1DOP	Tag-lite Delta opioid labeled cells	200 tests
C1SU1GLP1	Tag-lite Glucagon GLP1 cell line (HEK293)	1 each
61TK0BLE	TK Substrate-biotin	50 ug
62SAHZLC	Ultrapure SAM cofactor (2.4 umol)	2,4 umol

For more information, please visit: www.cisbio.eu

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