HTRF®, is the premier TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) technology on the market. TR-FRET technologies such as
HTRF® bring together the sensitivity of fluorescence with the homogeneous nature of FRET (Fluorescence Resonance Energy Transfer) and the low background of time resolution.
HTRF® uses two fluorophores, a donor and an acceptor dye, that transfer energy when in close proximity to each other. This creates a homogeneous assay format in which bound and unbound partners do not need to be separated as fluorescence emission from the acceptor is generated only upon binding.
HTRF® can be used in competitive and non-competitive formats and performed as cellular or biochemical assays in 96-, 384- and 1536-well plate formats. It has been applied to a variety of applications including GPCRs, kinases, epigenetics, biotherapeutics and quantification of a range of biomarkers including cytokines.
Bio-Connect Diagnostics supplies all
Cisbio HTRF® products in the Benelux. You can
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