Belgium

Luxembourg

Netherlands

ELISA / EIA

Assays

BPS Bioscience

Supplier

Research Use Only

Certification

Bio-Connect Life Sciences

Business unit

LysA™ Universal PARylation Assay workflow diagram.

Effect of the PARG inhibitor PDD00017273 on cellular PARylation levels in various cell lines.

82123 | LysA(tm) Universal PARylation Assay Kit | All BPS Bioscience products available at Bio-Connect

LysA(tm) Universal PARylation Assay Kit | 82123 | BPS Bioscience

LysA(tm) Universal PARylation Assay Kit

LysA™ Universal PARylation Assay Kit is a sandwich ELISA-based kit designed to measure and quantify the amount of total poly ADP-ribosylation present in cell extracts. The kit comes in a convenient 96-well format and contains all the reagents needed, including a PAR standard that allows to establish a standard curve for quantitative measurements. It contains enough antibodies, blocking buffer, and detection reagents to measure PARylation levels in cell extracts, in a 96 well plate format. It also includes cell lysates as controls for assay performance. Note: The assay is adequate for Poly-ADP-ribosylation detection only. The assay is linear in the 100 pM to 20 nM PARylation range. An anti-PAR antibody is used to coat the 96-well plate. Lysates from cells are added to the coated wells, so PAR (PARylated proteins) present in the cell lysates are captured by the antibody. This is followed by an incubation with an anti-PAR primary antibody and a secondary HRP-labeled antibody. Addition of a chemiluminescent HRP substrate provides a luminescence signal, which directly correlates with the amount of PAR present in the cell extracts

Tech support

News

Our company

Our people

Divisions

Vacatures

Events

Promotions

About us

Contact

Antibodies

Chemicals

Proteins / Signaling Molecules

(Cell) Culture

Products

Suppliers

Industries

Bio-Connect

Cancer

Epigenetics

Immunology and inflammation

Neuroscience

Virology

SARS-CoV-2

Research

Disclaimer

Privacy Statement

Terms and conditions

Are you looking for specific products, alternatives, documentation or need assistance with solving product related issues? Let our in-house technical support team help you.

We support your research

<p>For 15 years, we connect life science researchers specialized in the import and distribution of life science reagents from global suppliers.</p>

Support your research

DNA / RNA / Vectors

Cell Culture

Genome Editing and Engineering

Molecular Biology

Lysates / Extracts

Expression

Labware / Accesories

Body Products

Technique Specific Products

Arrays / Slides

Cancer research

Epigenetics research

Immunology and inflammation research

Neuroscience research

Virology research

Call +31 (0)26 326 44 50

ELISA / EIA

Didn't find what you were looking for?