LymphoTrack® Assays


Invivoscribe’s LymphoTrack Assays are used to identify and track clonal lymphocyte populations. These RUO assays, developed for use with the leading Next-Generation Sequencing (NGS) platforms, include optimized multiplex PCR master mixes with primers incorporating both platform specific adapters, and specimen tracking Seq ID tags for single step workflow. They also include a comprehensive bioinformatics package, enabling you to identify the DNA sequence, clonal prevalence, and V-J family identity for each gene rearrangement.

Key Benefits

• One-step PCR for streamlined workflow
• Identical workflow for clonal detection and MRD analysis
• Multiple indices for simultaneous testing of multiple samples with multiple assays
• Bioinformatics software for simple, automated visualization of data and analysis
• Unparalleled sensitivity of clonality detection

LymphoTrack® RUO assays are offered as kits for either the Illumina MiSeq® or Ion PGM™ platforms. See below for platform specific information.

Illumina MiSeq® »»

 

Ion PGM™ »»

The LymphoTrack MiSeq master mixes are designed with 24 TruSeq indices and adapters for the Illumina MiSeq instrument, allowing one-step PCR, pooling of amplicons, and loading onto the flowcell.

LymphoTrack IGH Assay - MiSeq
LymphoTrack IGK Assay - MiSeq
LymphoTrack TRG Assay - MiSeq
LymphoTrack IGH + TRG Assay - MiSeq
LymphoTrack IGH Somatic Hypermutation Assay - MiSeq
 The LymphoTrack PGM master mixes are designed with 12 Ion Xpress™ indices and adapters for the Ion PGM instrument, allowing one-step PCR, pooling of amplicons, and loading onto the chip.

LymphoTrack IGH Assay - PGM
LymphoTrack IGK Assay - PGM
LymphoTrack TRG Assay - PGM
LymphoTrack IGH + TRG Assay - PGM
LymphoTrack IGH Somatic Hypermutation Assay - PGM
 Read more »»  Read more »»

Workflow: A single-step PCR library preparation is followed by standard MiSeq or PGM sequencing. The proprietary LymphoTrack Bioinformatics Software (included in the assay kit) directly converts NGS data output to a format that allows you to visualize and analyze the prevalence or frequency distribution of the gene rearrangements and to identify specific DNA sequences. The software facilitates interpretation and subsequent tracking of clonal populations. With this state-of-the-art assay, the gene rearrangement status of each of the several million lymphocytes in blood or tissue samples can be identified and characterized.

V-J Sequence Frequencies
 V-J Usage

References

Targeted deep sequencing reveals clinically relevant subclonal IgHV rearrangements in chronic lymphocytic leukemia.
Stamatopoulos B, Timbs A, Bruce D, Smith T, Clifford R, Robbe P, Burns A, Vavoulis DV, Lopez L, Antoniou P, Mason J, Dreau H, Schuh A.

Detection of immunoglobulin heavy chain gene clonality by next-generation sequencing for minimal residual disease monitoring in B-Lymphoblastic Leukemia.
Saeam Shin, M.D., In Sik Hwang, M.S., Jieun Kim, M.D., Kyung-A Lee, M.D., Seung-Tae Lee, M.D., and Jong Rak Choi, M.D.

Clinical-scale rapid autologous BK-virus specific T cell line generation from kidney transplant recipients with active viremia for adoptive immunotherapy.
Lamarche C, Orio J, Georges-Tobar V, Pincez T, Goupil M, Dahmani A, Carli C C, Brasey A, Busque L, Delisle JS.

T and B cell clonal expansion in Ras associated lymphoproliferative disease (RALD) as revealed by next-generation sequencing.
Levy-Mendelovich S, Lev A, Rechavi E, Barel O, Golan H, Bielorai B, Neumann Y, Simon A, Somech R.



Source:
Product flyers: Portfolio for the Ion S5/PGMPortfolio for the MiSeq
Workflows: Ion S5/PGM and MiSeq assays.
Website: Invivoscribe

Related to:
Brands: Invivoscribe
News: LymphoTrack Assays for the Illumina MiSeq®,  LymphoTrack Assays for the Ion PGM™
Product groups: Assays