LymphoTrack® Dx Assays

Invivoscribe’s LymphoTrack® Dx Assays are used to identify and track clonal lymphocyte populations. These CE-IVD assays, developed for use with the leading Next-Generation Sequencing (NGS) platforms, include optimized multiplex PCR master mixes with primers incorporating platform specific adapters and specimen tracking sequencing identification tags for a one-step PCR workflow. A comprehensive bioinformatics software package is provided free of charge with purchase; enabling you to identify the DNA sequence, clonal prevalence, V-J family identity for each gene rearrangements, and with the IGH assays, the extent of IGHV somatic hypermutation (SHM). With this state-of-the-art assay, the gene rearrangement status of several million lymphocytes in blood, bone marrow, or tissue samples can be both identified and characterized.

Key Benefits

• Intended for identification of clonality in suspected lymphoproliferations
• One-step PCR for amplicon and library generation
• Configured with up to 24 indices for workflow flexibility
• Positive and negative controls included in kits
• Included bioinformatics software for easy analysis and interpretation
• Intended for identification of clonality in suspected lymphoproliferations

Simple Workflow

• Single-step PCR for library preparation
• Standard MiSeq or PGM sequencing
• NGS data allows direct visualization and analysis of the prevalence and distribution of gene rearrangements sorted and identified by their DNA sequence
• Proprietary software facilitates easy interpretation and subsequent tracking of clonal populations

Multiplexing Capabilities

• Kits include up to 24 indices to increase workflow flexibility
• Designed with the ability to pool multiple targets in a single sequencing run
• Decreased testing costs
​• Increased throughput capabilities

LymphoTrack Dx Software

• Analyze raw FASTQ files
• Fast computing algorithm - an average of 90-120 seconds per sample
• Ability to analyze sequences from different targets at the same time
• Data is analyzed directly onsite without the need for an internet connection
• Results are auto-formatted into 7 different graphs and tables

Data Interpretation

• Easy clinical interpretation for the evidence of clonality
• ​DNA sequence, frequency, length, and family directly revealed
• Somatic Hypermutation status calculated from IGHV sequencing data
• Backed by clinical studies
• Clonal sequences can be tracked in subsequent samples

Illumina MiSeq® »»


Ion PGM® »»

The LymphoTrack Dx MiSeq master mixes are designed with 24 TruSeq indices and adapters for the Illumina MiSeq instrument, allowing one-step PCR, pooling of amplicons, and loading onto the flowcell.

LymphoTrack Dx IGH FR1 Assay - MiSeq
LymphoTrack Dx IGK Assay - MiSeq
LymphoTrack Dx TRG Assay - MiSeq

 The LymphoTrack Dx PGM master mixes are designed with 12 Ion Xpress™ indices and adapters for the Ion PGM instrument, allowing one-step PCR, pooling of amplicons, and loading onto the chip.

LymphoTrack Dx IGH FR1 Assay - PGM
LymphoTrack Dx IGK Assay - PGM
LymphoTrack Dx TRG Assay - PGM



Targeted deep sequencing reveals clinically relevant subclonal IgHV rearrangements in chronic lymphocytic leukemia.
Stamatopoulos B, Timbs A, Bruce D, Smith T, Clifford R, Robbe P, Burns A, Vavoulis DV, Lopez L, Antoniou P, Mason J, Dreau H, Schuh A.

Detection of immunoglobulin heavy chain gene clonality by next-generation sequencing for minimal residual disease monitoring in B-Lymphoblastic Leukemia.
Saeam Shin, M.D., In Sik Hwang, M.S., Jieun Kim, M.D., Kyung-A Lee, M.D., Seung-Tae Lee, M.D., and Jong Rak Choi, M.D.

Clinical-scale rapid autologous BK-virus specific T cell line generation from kidney transplant recipients with active viremia for adoptive immunotherapy.
Lamarche C, Orio J, Georges-Tobar V, Pincez T, Goupil M, Dahmani A, Carli C C, Brasey A, Busque L, Delisle JS.

T and B cell clonal expansion in Ras associated lymphoproliferative disease (RALD) as revealed by next-generation sequencing.
Levy-Mendelovich S, Lev A, Rechavi E, Barel O, Golan H, Bielorai B, Neumann Y, Simon A, Somech R.

Related to:
Brands: Invivoscribe
News: LymphoTrack® Dx Assays for the Illumina MiSeq®LymphoTrack® Dx Assays for the Ion PGM®
Product groups: Assays