ProteoSpin Inclusion Body Protein Isolation Micro Kit

Catalog number: 10300
Brand: Norgen Biotek
Packing: 25 preps
Price: € 339.00
Expected delivery time: 7 days

Product specifications for - ProteoSpin Inclusion Body Protein Isolation Micro Kit

Product group: Molecular Biology
Category: Protein / lipid extraction / purification
Subcategory: Columns / resins
Datasheet: Datasheet
  Research Use Only
UNSPSC: 41116133
Scientific information: 
Scientific info: This Norgen Biotek kit provides everything required to isolate and purify inclusion body proteins from induced bacterial cultures. First a proprietary Cell Lysis Reagent is used to selectively lyse the cells and release inclusion bodies in their solid form. Next, inclusion bodies are dissolved and their contents released using the provided IB Solubilization Reagent. Inclusion body proteins are then further purified using spin columns for rapid and convenient buffer exchange and desalting. This kit provides a convenient way to screen recombinants prior to scaling up.

The process is efficient and streamlined and can process up to 12 samples in only 60 minutes. Each spin column is able to recover up to 50 µg of acidic or basic proteins. Purified recombinant proteins are then ready for SDS-PAGE, 2D gels, Western blots, Mass Spectrometry analysis, and other applications.
Additional information: 
Synonyms: 10300