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Active Macrophage Inflammatory Protein 1 Alpha (MIP1a)
Catalog number:
APA092HU61_50ug
Brand:
Cloud-Clone Corp.
Packing:
50 ug
Price:
On Request
Expected delivery time:
7 days
Quantity:
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General
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MIP-1a (macrophage inflammatory pro...
MIP-1a (macrophage inflammatory protein 1-alpha) also known as Chemokine (C-C motief) ligand 3 (CCL3), is a cytokine belonging to the CC chemokine family that is involved in the recruitment and activation of macrophages, monocytes and neutrophils. In this case, chemotaxis assay used 24-well microchemotaxis system was undertaken to evaluate the chemotactic effect of MIP-1a on the human monocytic cell line THP1. Briefly, THP1 cells were seeded into the upper chambers (100µl cell suspension, 106 cells/ml in RPMI 1640 with 0.5% FBS) and MIP-1a (100ng/mL, diluted in serum free RPMI 1640 ) was added in lower chamber with a polycarbonate filter (8µm pore size) used to separate the two compartments. After incubation at 37oC with 5% CO2 for 5h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×40) and the number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter).
By counting migrated cells in low chamber at high magnification (×400) randomly, it was shown that a mean of 41.2 THP1 cells/field migrated towards serum free RPMI 1640 medium with 100ng/mL MIP-1a, while only 3.6 THP1 cells/field migrated towards serum free RPMI 1640 medium. And the migrated THP1 cells in low chamber at low magnification (×40) was shown in Figure 1.
Product specifications for - Active Macrophage Inflammatory Protein 1 Alpha (MIP1a)
Overview:
Product group:
Proteins / Signaling Molecule
Category:
Chemokines / cytokines
Application:
Other Application
Application note:
Cell culture, Activity Assays, In vivo assays.
Modification:
N-terminal His Tag
Properties:
Purity:
> 95%
Molecular weight:
14kDa
Datasheet:
Datasheet
Research Use Only
UNSPSC:
12352202
Scientific information:
Scientific info:
Eukaryotic expression
Swiss prot ID:
P10147
Additional information:
Synonyms:
APA092HU61
