Wide range mouse Insulin ELISA Kit
ORB54821
Overview
- SupplierBiorbyt
- Product NameWide range mouse Insulin ELISA Kit
- Delivery Days Customer16
- ApplicationsELISA
- Applications SupplierELISA
- Assay Detection Range1-50 ng/ml
- CertificationResearch Use Only
- Scientific DescriptionThis assay is a two-site ELISA. The micro-plate is pre-coated with a monoclonal antibody against insulin. Standards and samples are added into the wells and co-incubated with a monoclonal antibody conjugated to horseradish peroxidase (HRP) enzyme. After wash step to remove any unbound substances, TMB substrate is added and colour develops in proportion to the amount of insulin bound initially. The assay is stopped and the optical density of the wells determined using a micro-plate reader. Since the increases in absorbance are directly proportional to the amount of captured insulin, the unknown sample concentration can be interpolated from a reference curve included in each assay.
- ReactivityMouse
- Storage Instruction2°C to 8°C
- UNSPSC41116133
References
- Low ambient temperature during early postnatal development fails to cause a permanent induction of brown adipocytes. Chabowska-Kita A et al., 2015 Aug, FASEB JRead more