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Anti-peeling Slide
Product group Antibodies
Boster Bio
Anti-peeling SlideAR1065
Western blot analysis of Phospho-RPA2 (T21) expression in HeLa cell lysate treated with Calyculin A.
Product group Antibodies
Boster Bio
Anti-Phospho-RPA2 (T21) Rabbit Monoclonal AntibodyP02067-1
Western blot analysis of Phospho-CDC37 (S13) expression in (1) Jurkat cell lysate treated with Alkaline Phosphatase; (2) Jurkat cell lysate (P02169). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC37 monoclonal antibody (Catalog # P02169) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDC37
Product group Antibodies
Boster Bio
Anti-Phospho-CDC37 (S13) Rabbit Monoclonal AntibodyP02169
Western blot analysis of Phospho-CrkII (Tyr221) expression in (1) K562 cell lysate treated with AP; (2) K562 cell lysate (P02533). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRK monoclonal antibody (Catalog # P02533) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CRK
Product group Antibodies
Boster Bio
Anti-Phospho-CrkII (Tyr221) Rabbit Monoclonal AntibodyP02533
Immunohistochemical analysis of paraffin-embedded mouse brain, using Phospho-PAK1/2/3 (S144+S141+S139) Antibody(P03124) PAK3 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PAK3 Antibody (P03124)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-Phospho-PAK1/2/3 (S144+S141+S139) Rabbit Monoclonal AntibodyP03124
Western blot analysis of GSK3 alpha/ beta phosphorylation expression in 293 cell lysate treated with AP (P03152-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSK3A monoclonal antibody (Catalog # P03152-1) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GSK3A
Product group Antibodies
Boster Bio
Anti-Phospho-GSK3 (alpha + beta) (Y216 + Y279) Rabbit Monoclonal AntibodyP03152-1
Western blot analysis of Phospho-SHIP (Y1020) expression in (1) Raji cell lysate; (2) Raji cell treated with pervanadate lysate (P03358-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-INPP5D monoclonal antibody (Catalog # P03358-1) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for INPP5D
Product group Antibodies
Boster Bio
Anti-Phospho-SHIP (Y1020) Rabbit Monoclonal AntibodyP03358-1
Western blot analysis of Phospho-Synapsin I (S9) expression in (1) Human brain lysate; (2) Human brain lysate treated with AP (P03794). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYN1 monoclonal antibody (Catalog # P03794) overnight at 4 °C; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SYN1
Product group Antibodies
Boster Bio
Anti-Phospho-Synapsin (Ser549) AntibodyP03794
Western blot analysis of Phospho-PKA R2 (Ser99) in K562 cell lysate (P03911). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKAR2A monoclonal antibody (Catalog # P03911) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRKAR2A
Product group Antibodies
Boster Bio
Anti-Phospho-PKA R2 (S99) Rabbit Monoclonal AntibodyP03911
Immunohistochemical analysis of paraffin-embedded human colon cancer, using Phospho-EIF2S1 (S51) Antibody.
Product group Antibodies
Boster Bio
Anti-Phospho-EIF2S1 (S51) Rabbit Monoclonal AntibodyP04387
Western blot analysis of Phospho-JunD (S255) expression in (1) HeLa cell lysate treated with Alkaline Phosphatase; (2) HeLa cell lysate (P05609). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JUND monoclonal antibody (Catalog # P05609) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JUND
Product group Antibodies
Boster Bio
Anti-Phospho-JunD (S255) Rabbit Monoclonal AntibodyP05609
Immunohistochemical analysis of paraffin-embedded mouse colon, using Phospho-Histone H1.4 (T17) Antibody.
Product group Antibodies
Boster Bio
Anti-Phospho-Histone H1.4 (T17) Rabbit Monoclonal AntibodyP06652
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