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Search results: 10-1113/currency/EUR

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Product group Antibodies
Nordic-MUbio
ApplicationsWestern Blot, ELISA
ReactivityHuman
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Product group Antibodies
Nordic-MUbio
ApplicationsWestern Blot, ELISA
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Product group Proteins / Signaling Molecules
Cygnus Technologies
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Product group Chemicals
MedChem Express
CAS Number1113-60-6
Estimated Purity95%
Molecular Weight104.06
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Product group Chemicals
MedChem Express
CAS Number1113-59-3
Estimated Purity98%
Molecular Weight166.96
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Western blot analysis of Histone H3 expression in (1) HeLa cell lysate; (2) 3T3 cell lysate (M12477-10). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1H3A monoclonal antibody (Catalog # M12477-10) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIST1H3A
Product group Antibodies
Boster Bio
Product group Chemicals
MedChem Express
CAS Number1113-83-3
Estimated Purity98%
Molecular Weight325.27
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Product group Chemicals
FUJIFILM Wako Chemicals
Product group Proteins / Signaling Molecules
ZeptoMetrix
Product group Proteins / Signaling Molecules
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Product group Assays
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Product group Assays
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