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Search results: CD53

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Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in germinal center cells.
Product group Antibodies
Atlas Antibodies
Anti-CD53HPA047216
ApplicationsImmunoHistoChemistry
ReactivityHuman
TargetCD53
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Product group Antibodies
GeneTex
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
ReactivityHuman
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GTX76437 FACS Image
Product group Antibodies
GeneTex
ApplicationsFlow Cytometry
ReactivityHuman
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Product group Antibodies
Absolute Antibody
ApplicationsImmunoHistoChemistry
ReactivityRat
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Product group Antibodies
Absolute Antibody
Anti-CD53 [OX-44]Ab00585-23.0
ApplicationsImmunoHistoChemistry
ReactivityRat
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Product group Antibodies
Absolute Antibody
ApplicationsImmunoHistoChemistry
ReactivityRat
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Product group Antibodies
Absolute Antibody
ApplicationsImmunoHistoChemistry
ReactivityRat
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Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
ReactivityMouse
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Product group Antibodies
LifeSpan BioSciences
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
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Product group Antibodies
LifeSpan BioSciences
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
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Western blot analysis of CD53 expression in Jurkat cell lysate using anti-CD53 antibody (M06249). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD53 antigen affinity purified polyclonal antibody (Catalog # M06249) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for CD53.
Product group Antibodies
Boster Bio
Product group Antibodies
MyBioSource
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