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Search results: CHI3L1

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Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot
ReactivityHuman
TargetCHI3L1
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Product group Antibodies
GeneTex
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse
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Whole cell extract (30 microg) was separated by 10% SDS-PAGE, and the membrane was blotted with CHI3L1 antibody (GTX135636) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
GeneTex
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Product group Proteins / Signaling Molecules
MyBioSource
CHI3L1, HumanMBS8575304
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Product group Antibodies
GeneTex
ApplicationsELISA
ReactivityHuman
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GTX67296-pro Image
Product group Proteins / Signaling Molecules
GeneTex
Protein IDP36222
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Western blot analysis of CHI3L1 using anti-CHI3L1 antibody (A01283-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse RAW264.7 whole cell lysates, Lane 2: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHI3L1 antigen affinity purified polyclonal antibody (Catalog # A01283-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHI3L1 at approximately 43KD. The expected band size for CHI3L1 is at 43KD.
Product group Antibodies
Boster Bio
Product group Antibodies
Biorbyt
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCHI3L1
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Product group Antibodies
Atlas Antibodies
Anti-CHI3L1HPA077365
ApplicationsImmunoCytoChemistry
ReactivityHuman
TargetCHI3L1
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Product group Antibodies
Atlas Antibodies
Anti-CHI3L1AMAB91777
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetCHI3L1
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Product group Antibodies
Atlas Antibodies
Anti-CHI3L1AMAB91778
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetCHI3L1
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Product group Antibodies
Reddot Biotech
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetChil1
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