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Search results: FGF23

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Product group Proteins / Signaling Molecules
GeneTex
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Product group Antibodies
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
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Western blot analysis of FGF23 using anti-FGF23 antibody (A00478-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysate, Lane 2: rat PC-12 whole cell lysate, Lane 3: mouse testis tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF23 antigen affinity purified polyclonal antibody (Catalog # A00478-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF23 at approximately 35KD. The expected band size for FGF23 is at 28KD.
Product group Antibodies
Boster Bio
Product group Antibodies
Everest Biotech
ApplicationsWestern Blot, ELISA
ReactivityHuman
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GTX00257-pro Image
Product group Proteins / Signaling Molecules
GeneTex
ApplicationsFunctional Assay
Protein IDQ9GZV9
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GTX00295-pro Image
Product group Proteins / Signaling Molecules
GeneTex
ApplicationsFunctional Assay
Protein IDQ9EPC2
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Product group Proteins / Signaling Molecules
LifeSpan BioSciences
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Western blot analysis of FGF 23 using anti-FGF 23 antibody (PB9868).
Product group Antibodies
Boster Bio
Product group Proteins / Signaling Molecules
LifeSpan BioSciences
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Product group Proteins / Signaling Molecules
LifeSpan BioSciences
  • SizePrice
Product group Proteins / Signaling Molecules
LifeSpan BioSciences
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot
ReactivityHuman
  • SizePrice