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Search results: ICAM1

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Product group Assays
DLdevelop
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Product group Assays
DLdevelop
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Product group Assays
DLdevelop
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Product group Assays
DLdevelop
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Product group Assays
DLdevelop
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Product group Assays
DLdevelop
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Product group Antibodies
MyBioSource
ICAM1/CD54MBS8577788
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GTX32663 ICC/IF Image
Product group Antibodies
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
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GTX50456 WB Image
Product group Antibodies
GeneTex
ApplicationsWestern Blot
ReactivityHuman
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GTX66707 WB Image
Product group Antibodies
GeneTex
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
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Western blot analysis of ICAM1 using anti-ICAM1 antibody (A00171). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysate, Lane 2: rat thymus tissue lysate, Lane 3: rat RH35 cell lysate, Lane 4: mouse spleen tissue lysate, Lane 5: mouse thymus tissue lysate, Lane 6: mouse HEPA1-6 cell lysate, Lane 7: mouse heart tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ICAM1 antigen affinity purified polyclonal antibody (Catalog # A00171) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ICAM1 at approximately 90KD. The expected band size for ICAM1 is at 58KD.
Product group Antibodies
Boster Bio
Product group Antibodies
Everest Biotech
ApplicationsImmunoFluorescence, ELISA
ReactivityHuman
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