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Search results: RIA

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Product group Chemicals
MP Biomedicals (Life Sciences)
CAS Number9048-46-8
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Product group Chemicals
MP Biomedicals (Life Sciences)
CAS Number9048-46-8
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Product group Chemicals
MP Biomedicals (Life Sciences)
CAS Number9048-46-8
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Product group Chemicals
MP Biomedicals (Life Sciences)
CAS Number9048-46-8
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Product group Diagnostic
Antibodies.com
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Product group Proteins / Signaling Molecules
Biorbyt
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Western blot analysis of APBB1IP using anti-APBB1IP antibody (PA1960 ). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: Jurkat whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: U937 whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APBB1IP antigen affinity purified polyclonal antibody (Catalog # PA1960 ) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APBB1IP at approximately 110-120KD. The expected band size for APBB1IP is at 73KD.
Product group Antibodies
Boster Bio
GTX62255 IHC-P Image
Product group Antibodies
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse
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Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetAPBB1IP
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Product group Antibodies
LifeSpan BioSciences
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
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Product group Antibodies
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA
ReactivityHuman, Mouse
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