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Search results: ZIC1

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Product group Proteins / Signaling Molecules
MyBioSource
Protein IDQ15915
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Product group Antibodies
MyBioSource
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Product group Proteins / Signaling Molecules
MyBioSource
Protein IDQ15915
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Product group DNA / RNA / Vectors
Sino Biological
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Product group Antibodies
Biorbyt
ZIC1 antibodyORB574696
ApplicationsWestern Blot
ReactivityHuman
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Product group Assays
LifeSpan BioSciences
ReactivityHuman, Mouse
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Product group Antibodies
MyBioSource
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Product group Antibodies
Cusabio
ZIC1 AntibodyCSB-PA795975
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
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Product group Antibodies
Biorbyt
ZIC1 antibodyORB380157
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse
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Product group Assays
Biomatik
Assay Sample TypeResearchers plate their cell line of choice
ReactivityHuman, Mouse
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Western blot analysis of ZIC1 expression in SW480 cell lysate using anti-ZIC1 antibody (M05537). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZIC1 antigen affinity purified polyclonal antibody (Catalog # M05537) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
Boster Bio
Product group Antibodies
MyBioSource
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