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Antibodies

We offer one of the most comprehensive portfolios of antibodies. This includes monoclonal and polyclonal primary, secondary, conjugated, phospho-specific, functional, (isotype) controls, tagged and antibody pairs. In addition, we offer custom antibody services from several manufacturers.

The antibodies are generated in various hosts and react to antigens of different species like human, mouse, rat, rabbit or zebrafish. The antibodies are validated for multiple applications, including immunohistochemistry, western blot, immunoprecipitation, ELISA and flow cytometry, to ensure reliable performance for your research needs.

If you need help finding the right antibodies for your research, please use our antibody request form.

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Immunohistochemical analysis of paraffin-embedded human kidney, using Phospho-Akt(Ser473) Antibody.
Product group Antibodies
Boster Bio
Western blot analysis of Phospho-c-Myc (T58 + S62) in HEK293 cell lysate treated with EGF (P00026-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYC monoclonal antibody (Catalog # P00026-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYC
Product group Antibodies
Boster Bio
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-c-Myc (S62) Antibody.
Product group Antibodies
Boster Bio
Western blot analysis of Phospho-c-Myc (T58) expression in (1) HeLa cell lysate; (2) HeLa cell lysate treated with Calyculin A and Okadaic Acid.
Product group Antibodies
Boster Bio
Western blot analysis of Phospho-STAT1 (Y701) expression in (1) A431 cell lysate; (2) A431 lysate cell treated with EGF.
Product group Antibodies
Boster Bio
Immunohistochemical analysis of paraffin-embedded mouse spleen, using Phospho-Retinoblastoma (S807) Antibody.
Product group Antibodies
Boster Bio
Western blot analysis of Phospho-ER alpha (S118) expression in (1) MCF7 cell lysate; (2) MCF7 cell lysate treated with b-Estradiol and EGF.
Product group Antibodies
Boster Bio
Immunofluorescent analysis of A549 cells treated with TGFβ, using Phospho-Smad3 (S423 + S425) Antibody.
Product group Antibodies
Boster Bio
Western blot analysis of Phospho-B Raf (T401) expression in (1) PC-12 cell lysate; (2) PC-12 cell lysate treated with TPA.
Product group Antibodies
Boster Bio
Western blot analysis of Nrf2 phosphorylation expression in HepG2 cell lysate.
Product group Antibodies
Boster Bio
Western blot analysis of Phospho-Smad2 (S250) expression in (1) HeLa cell treated with alkaline phosphatase lysate; (2) HeLa cell lysate (P00090-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD2 monoclonal antibody (Catalog # P00090-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAD2
Product group Antibodies
Boster Bio
Western blot analysis of Phospho-Smad2 (S255) expression in HeLa cell treated with Okadaic acid and Calyculin A lysate (P00090). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD2 monoclonal antibody (Catalog # P00090) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAD2
Product group Antibodies
Boster Bio