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Proteins / Signaling Molecules

This product group contains both native proteins and recombinant proteins expressed and purified from bacterial, insect and mammalian expression systems. Bio-Connect also offers fusion proteins, peptides, enzymes, bacterial and viral proteins, hormones and growth factors. Furthermore, it includes chemokines and cytokines, collagenases, albumins, globulins, blood/plasma related proteins and more.

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Western blot analysis of cleaved Caspase-9 Antibody expression in HeLa cell lysate treated with staurosporine (M00080-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP9 monoclonal antibody (Catalog # M00080-2) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CASP9
Product group Proteins / Signaling Molecules
Boster Bio
Anti-cleaved Caspase-9 Rabbit Monoclonal AntibodyM00080-2
Immunohistochemical analysis of paraffin-embedded human liver cancer, using Caspase-9 Antibody(M00080-3) CASP9 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CASP9 Antibody (M00080-3)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Proteins / Signaling Molecules
Boster Bio
Anti-Caspase-9 Rabbit Monoclonal AntibodyM00080-3
Western blot analysis of Caspase-9 expression in HeLa cell lysate.
Product group Proteins / Signaling Molecules
Boster Bio
Anti-Caspase-9 Rabbit Monoclonal AntibodyM00080
Anti-SERCA1 ATPase antibody, PA1719, Western blotting Lane 1: Rat Skeletal Muscle Tissue Lysate Lane 2: PANC Cell Lysate Lane 3: U87 Cell Lysate
Product group Proteins / Signaling Molecules
Boster Bio
Anti-SERCA1 ATPase AntibodyPA1719
Protein IDO14983
Anti-SERCA2 ATPase antibody, PA1720, IHC(P) IHC(P): Human Mammary Cancer Tissue
Product group Proteins / Signaling Molecules
Boster Bio
Anti-SERCA2 ATPase AntibodyPA1720
Protein IDP16615
Western blot analysis of PTGS2 using anti-PTGS2 antibody (PA1725).
Product group Proteins / Signaling Molecules
Boster Bio
Anti-COX2/Cyclooxygenase 2 AntibodyPA1725
Protein IDP35354
Anti-Caspase-8(p18) antibody, PA1735, Western blotting All lanes: Anti Caspase-8(p18) (PA1735) at 0.5ug/ml Lane 1: A549 Whole Cell Lysate at 40ug Lane 2: HELA Whole Cell Lysate at 40ug Predicted bind size: 18KD Observed bind size: 18KD
Product group Proteins / Signaling Molecules
Boster Bio
Anti-Caspase-8 (p18) AntibodyPA1735
Protein IDQ14790
Anti-Caspase-9 antibody, PA1595, Western blotting All lanes: Anti Caspase-9 (PA1595) at 0.5ug/ml Lane 1: SMMC Whole Cell Lysate at 40ug Lane 2: MCF-7 Whole Cell Lysate at 40ug Lane 3: CEM Whole Cell Lysate at 40ug Lane 4: JURKAT Whole Cell Lysate at 40ug Lane 5: RAJI Whole Cell Lysate at 40ug Lane 6: HELA Whole Cell Lysate at 40ug Predicted bind size: 35KD Observed bind size: 35KD
Product group Proteins / Signaling Molecules
Boster Bio
Anti-Caspase-9 AntibodyPA1595
Protein IDP55211
Anti-12 Lipoxygenase antibody, PA1485-1, Western blotting All lanes: Anti ANOX12 (PA1485-1) at 0.5ug/ml
Product group Proteins / Signaling Molecules
Boster Bio
Anti-12 Lipoxygenase AntibodyPA1485-1
Protein IDP0CG47
Western blot analysis of Alpha Amylase 1 using anti-Alpha Amylase 1 antibody (PA1486). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PANC-1 whole cell lysates, Lane 2: rat pancreas tissue lysates, Lane 3: mouse pancreas tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha Amylase 1 antigen affinity purified polyclonal antibody (Catalog # PA1486) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha Amylase 1 at approximately 55-58KD. The expected band size for Alpha Amylase 1 is at 58KD.
Product group Proteins / Signaling Molecules
Boster Bio
Anti-Alpha Amylase 1 AntibodyPA1486
Protein IDP04745
Anti-Aromatase antibody, PA1489, Western blotting Lane 1: Human Placenta Tissue Lysate Lane 2: Human Placenta Tissue Lysate
Product group Proteins / Signaling Molecules
Boster Bio
Anti-Aromatase AntibodyPA1489
Protein IDP11511
Anti-Caspase 4 antibody, PA1490, Western blotting All lanes: Anti Caspase 4 (PA1490) at 0.5ug/ml
Product group Proteins / Signaling Molecules
Boster Bio
Anti-Caspase 4 AntibodyPA1490
Protein IDP49662
34567

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